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. 1983 Dec;80(23):7173–7177. doi: 10.1073/pnas.80.23.7173

Spatial correlation between primary redox components in reaction centers of Rhodopseudomonas sphaeroides measured by two electrical methods in the nanosecond range

H-W Trissl 1
PMCID: PMC390016  PMID: 16593393

Abstract

Relative distances between the the primary donor P, the intermediary pheophytin acceptor H, and the iron-quinone acceptor Q of bacterial reaction centers were determined by recording laser flash-induced photovoltages in two experimental systems with nanosecond time resolution. In one system a suspension of chromatophores was subjected to a light gradient and in the other system chromatophores were spread at a heptane/water interface. The 10-ns back reaction occurring in reaction centers with reduced Q could be time resolved. The initial photovoltage amplitude under conditions in which the charge separation proceeded up to the state [P+H-] was about ⅔ of that when it proceeded up to the state [P+HQ-]. If the amplitude of the photovoltage is considered to be proportional to the spatial displacement of charges, this result means that pheophytin lies closer to Q than to P.

Keywords: photosynthesis, charge separation, electron transfer, light gradient, capacitative electrode

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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