Abstract
A model is presented for the interference of GTP hydrolysis in the mechanism of microtubule assembly. This model is suggested by previous results showing that both GTP and GDP are present at microtubule ends because of GTP hydrolysis and that tubulin does not bind to a GDP-bound end. The analytical theory developed here is aimed at calculation of the steady-state subunit flux at one end of the polymer. The GTP/GDP features just mentioned result in a nonlinear plot of the flux versus tubulin concentration. Microtubules are predicted to exhibit a different kinetic behavior below and above the critical concentration, which can be considered as a transition between two regimes.
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Selected References
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- Carlier M. F. Guanosine-5'-triphosphate hydrolysis and tubulin polymerization. Review article. Mol Cell Biochem. 1982 Sep 3;47(2):97–113. doi: 10.1007/BF00234410. [DOI] [PubMed] [Google Scholar]
- Carlier M. F., Pantaloni D. Kinetic analysis of guanosine 5'-triphosphate hydrolysis associated with tubulin polymerization. Biochemistry. 1981 Mar 31;20(7):1918–1924. doi: 10.1021/bi00510a030. [DOI] [PubMed] [Google Scholar]
- David-Pfeuty T., Erickson H. P., Pantaloni D. Guanosinetriphosphatase activity of tubulin associated with microtubule assembly. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5372–5376. doi: 10.1073/pnas.74.12.5372. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hill T. L., Kirschner M. W. Bioenergetics and kinetics of microtubule and actin filament assembly-disassembly. Int Rev Cytol. 1982;78:1–125. [PubMed] [Google Scholar]
- Pardee J. D., Spudich J. A. Mechanism of K+-induced actin assembly. J Cell Biol. 1982 Jun;93(3):648–654. doi: 10.1083/jcb.93.3.648. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wegner A. Head to tail polymerization of actin. J Mol Biol. 1976 Nov;108(1):139–150. doi: 10.1016/s0022-2836(76)80100-3. [DOI] [PubMed] [Google Scholar]
- Weisenberg R. C., Deery W. J., Dickinson P. J. Tubulin-nucleotide interactions during the polymerization and depolymerization of microtubules. Biochemistry. 1976 Sep 21;15(19):4248–4254. doi: 10.1021/bi00664a018. [DOI] [PubMed] [Google Scholar]