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. 1983 Nov;80(22):6795–6798. doi: 10.1073/pnas.80.22.6795

Alteration and activation of sequence-specific cleavage of DNA by bleomycin in the presence of the antitumor drug cis-diamminedichloroplatinum(II).

P K Mascharak, Y Sugiura, J Kuwahara, T Suzuki, S J Lippard
PMCID: PMC390072  PMID: 6196777

Abstract

The antitumor drug cis-diamminedichloroplatinum(II) dramatically alters the sequence-specific cleavage of the bleomycin A2-iron(II)-O2 system. Preferred bleomycin cleavage sites adjacent to oligo(dG) regions on two restriction fragments of plasmid pBR322 DNA were masked by pretreatment with cis-diamminedichloroplatinum(II). trans-Diamminedichloroplatinum(II), which is inactive as an antitumor drug, showed similar but not identical behavior. The DNA-cleaving activity of bleomycin was substantially modified by cis-diamminedichloroplatinum(II), and a number of specific new cutting sites in guanine-rich parts of the sequence were activated by both isomers of the platinum complex. The results further emphasize the possibility that the synergism found when cis-diamminedichloroplatinum(II) and bleomycin are used in combination chemotherapy may be due to interactions at the level of DNA-drug binding.

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Selected References

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  1. Bajetta E., Rovej R., Buzzoni R., Vaglini M., Bonadonna G. Treatment of advanced malignant melanoma with vinblastine, bleomycin, and cisplatin. Cancer Treat Rep. 1982 Jun;66(6):1299–1302. [PubMed] [Google Scholar]
  2. Brouwer J., Fichtinger-Schepman A. M., van de Putte P., Reedijk J. Influence of temperature on platinum binding to DNA, cell killing, and mutation induction in Escherichia coli K-12 cells treated with cis-diamminedichloroplatinum(II). Cancer Res. 1982 Jun;42(6):2416–2419. [PubMed] [Google Scholar]
  3. Conner B. N., Takano T., Tanaka S., Itakura K., Dickerson R. E. The molecular structure of d(ICpCpGpG), a fragment of right-handed double helical A-DNA. Nature. 1982 Jan 28;295(5847):294–299. doi: 10.1038/295294a0. [DOI] [PubMed] [Google Scholar]
  4. D'Andrea A. D., Haseltine W. A. Sequence specific cleavage of DNA by the antitumor antibiotics neocarzinostatin and bleomycin. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3608–3612. doi: 10.1073/pnas.75.8.3608. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Davis H. L., Jr, von Hoff D. D., Henney J. E., Rozencweig M. The role of antitumor antibiotics in current oncologic practice. Cancer Chemother Pharmacol. 1978;1(2):83–90. doi: 10.1007/BF00254041. [DOI] [PubMed] [Google Scholar]
  6. Fichtinger-Schepman A. M., Lohman P. H., Reedijk J. Detection and quantification of adducts formed upon interaction of diamminedichloroplatinum (II) with DNA, by anion-exchange chromatography after enzymatic degradation. Nucleic Acids Res. 1982 Sep 11;10(17):5345–5356. doi: 10.1093/nar/10.17.5345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Girault J. P., Chottard J. C., Guittet E. R., Lallemand J. Y., Huynh-Dinh T., Igolen J. Specific platinum chelation by the guanines of the deoxyhexanucleotide d(T-G-G-C-C-A) upon reaction with cis-[Pt(NH3)2(H2O)2](NO3)2. Biochem Biophys Res Commun. 1982 Dec 31;109(4):1157–1163. doi: 10.1016/0006-291x(82)91898-8. [DOI] [PubMed] [Google Scholar]
  8. Kross J., Henner W. D., Hecht S. M., Haseltine W. A. Specificity of deoxyribonucleic acid cleavage by bleomycin, phleomycin, and tallysomycin. Biochemistry. 1982 Aug 31;21(18):4310–4318. doi: 10.1021/bi00261a021. [DOI] [PubMed] [Google Scholar]
  9. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  10. Mirabelli C. K., Beattie W. G., Huang C. H., Prestayko A. W., Crooke S. T. Comparison of the sequences at specific sites on DNA cleaved by the antitumor antibiotics talisomycin and bleomycin. Cancer Res. 1982 Apr;42(4):1399–1404. [PubMed] [Google Scholar]
  11. Perry D. J., Weltz M. D., Brown A. W., Jr, Henderson R. L., Neglia W. J., Berenberg J. L. Vinblastine, bleomycin and cisplatin for recurrent or metastatic squamous cell carcinoma of the head and neck. Cancer. 1982 Dec 1;50(11):2257–2260. doi: 10.1002/1097-0142(19821201)50:11<2257::aid-cncr2820501104>3.0.co;2-0. [DOI] [PubMed] [Google Scholar]
  12. Roberts J. J., Thomson A. J. The mechanism of action of antitumor platinum compounds. Prog Nucleic Acid Res Mol Biol. 1979;22:71–133. doi: 10.1016/s0079-6603(08)60799-0. [DOI] [PubMed] [Google Scholar]
  13. Shakked Z., Rabinovich D., Cruse W. B., Egert E., Kennard O., Sala G., Salisbury S. A., Viswamitra M. A. Crystalline A-dna: the X-ray analysis of the fragment d(G-G-T-A-T-A-C-C). Proc R Soc Lond B Biol Sci. 1981 Nov 24;213(1193):479–487. doi: 10.1098/rspb.1981.0076. [DOI] [PubMed] [Google Scholar]
  14. Sugiura Y., Suzuki T. Nucleotide sequence specificity of DNA cleavage by iron-bleomycin. Alteration on ethidium bromide-, actinomycin-, and distamycin-intercalated DNA. J Biol Chem. 1982 Sep 25;257(18):10544–10546. [PubMed] [Google Scholar]
  15. Sugiura Y., Suzuki T., Otsuka M., Kobayashi S., Ohno M., Takita T., Umezawa H. Synthetic analogues and biosynthetic intermediates of bleomycin. Metal-binding, dioxygen interaction, and implication for the role of functional groups in bleomycin action mechanism. J Biol Chem. 1983 Jan 25;258(2):1328–1336. [PubMed] [Google Scholar]
  16. Sutcliffe J. G. Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):77–90. doi: 10.1101/sqb.1979.043.01.013. [DOI] [PubMed] [Google Scholar]
  17. Takeshita M., Grollman A. P., Ohtsubo E., Ohtsubo H. Interaction of bleomycin with DNA. Proc Natl Acad Sci U S A. 1978 Dec;75(12):5983–5987. doi: 10.1073/pnas.75.12.5983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Takeshita M., Kappen L. S., Grollman A. P., Eisenberg M., Goldberg I. H. Strand scission of deoxyribonucleic acid by neocarzinostatin, auromomycin, and bleomycin: studies on base release and nucleotide sequence specificity. Biochemistry. 1981 Dec 22;20(26):7599–7606. doi: 10.1021/bi00529a039. [DOI] [PubMed] [Google Scholar]
  19. Tullius T. D., Lippard S. J. Ethidium bromide changes the nuclease-sensitive DNA binding sites of the antitumor drug cis-diamminedichloroplatinum(II). Proc Natl Acad Sci U S A. 1982 Jun;79(11):3489–3492. doi: 10.1073/pnas.79.11.3489. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wang A. H., Fujii S., van Boom J. H., Rich A. Molecular structure of the octamer d(G-G-C-C-G-G-C-C): modified A-DNA. Proc Natl Acad Sci U S A. 1982 Jul;79(13):3968–3972. doi: 10.1073/pnas.79.13.3968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wittes R. E., Brescia F., Young C. W., Magill G. B., Golbey R. B., Krakoff I. H. Combination chemothereapy with cis-diamminedichloroplatinum (II) and bleomycin in tumors of the head and neck. Oncology. 1975;32(5-6):202–207. doi: 10.1159/000225069. [DOI] [PubMed] [Google Scholar]

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