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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1983 Nov;80(22):6912–6916. doi: 10.1073/pnas.80.22.6912

Gene conversion and associated reciprocal recombination are separable events in vegetative cells of Saccharomyces cerevisiae.

H Roman, F Fabre
PMCID: PMC390096  PMID: 6359159

Abstract

Mitotic gene conversion occurs in both the G1 and G2 phases of cell growth. We postulate that the DNA strand or strands that connect the duplexes in G1 as a consequence of strand transfer are cleaved by an endonuclease. When this takes place in G1, crossing-over occurs in G2 at a frequency that is higher than would be expected from independent events. Conversion and associated reciprocal recombination are therefore separable with respect to the stage in the cell cycle when each occurs and possibly with respect to mechanism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Davidse L. C., Flach W. Differential binding of methyl benzimidazol-2-yl carbamate to fungal tubulin as a mechanism of resistance to this antimitotic agent in mutant strains of Aspergillus nidulans. J Cell Biol. 1977 Jan;72(1):174–193. doi: 10.1083/jcb.72.1.174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Esposito M. S. Evidence that spontaneous mitotic recombination occurs at the two-strand stage. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4436–4440. doi: 10.1073/pnas.75.9.4436. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fabre F. Induced intragenic recombination in yeast can occur during the G1 mitotic phase. Nature. 1978 Apr 27;272(5656):795–798. doi: 10.1038/272795a0. [DOI] [PubMed] [Google Scholar]
  4. Golin J. E., Esposito M. S. Mitotic recombination: mismatch correction and replicational resolution of Holliday structures formed at the two strand stage in Saccharomyces. Mol Gen Genet. 1981;183(2):252–263. doi: 10.1007/BF00270626. [DOI] [PubMed] [Google Scholar]
  5. KISSANE J. M., ROBINS E. The fluorometric measurement of deoxyribonucleic acid in animal tissues with special reference to the central nervous system. J Biol Chem. 1958 Jul;233(1):184–188. [PubMed] [Google Scholar]
  6. MESELSON M., WEIGLE J. J. Chromosome brekage accompanying genetic recombination in bacteriophage. Proc Natl Acad Sci U S A. 1961 Jun 15;47:857–868. doi: 10.1073/pnas.47.6.857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Meselson M. S., Radding C. M. A general model for genetic recombination. Proc Natl Acad Sci U S A. 1975 Jan;72(1):358–361. doi: 10.1073/pnas.72.1.358. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Sigal N., Alberts B. Genetic recombination: the nature of a crossed strand-exchange between two homologous DNA molecules. J Mol Biol. 1972 Nov 28;71(3):789–793. doi: 10.1016/s0022-2836(72)80039-1. [DOI] [PubMed] [Google Scholar]
  9. Wildenberg J. The relation of mitotic recombination to DNA replication in yeast pedigrees. Genetics. 1970 Oct;66(2):291–304. doi: 10.1093/genetics/66.2.291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Williamson D. H., Fennell D. J. The use of fluorescent DNA-binding agent for detecting and separating yeast mitochondrial DNA. Methods Cell Biol. 1975;12:335–351. doi: 10.1016/s0091-679x(08)60963-2. [DOI] [PubMed] [Google Scholar]
  11. Wood J. S., Hartwell L. H. A dependent pathway of gene functions leading to chromosome segregation in Saccharomyces cerevisiae. J Cell Biol. 1982 Sep;94(3):718–726. doi: 10.1083/jcb.94.3.718. [DOI] [PMC free article] [PubMed] [Google Scholar]

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