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. 2013 Dec 2;123(4):582–589. doi: 10.1182/blood-2013-05-505123

Figure 1.

Figure 1

Factor-IX Gla/Ds Red expression system. (A) Vector with doxycycline-inducible bidirectional promoter directing expression of cytoplasmic DsRed to assess induction, and chimeric factor-IX Gla domain-containing protein (F9CH) to measure carboxylation activity. Tet-On cells containing this vector are referred to as the “C3” cell line. (B) Chimeric factor-IX Gla protein contains prothrombin signal and pre-pro-peptide directing γ-carboxylation of the factor-IX Gla domain. APC-labeled antibody (GMA-001) specifically binds the γ-carboxylated factor-IX Gla domain.