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. 2012 May 10;1(2):127–140. doi: 10.3390/cells1020127

Figure 2.

Figure 2

Functional heterogeneity of antigen specific immunity at the single cell level indicates IFN-γ producing memory cells usually do not produce IL-2. PBMC from a representative subject were challenged with mumps-antigen (1/80 dilution) in 20 h culture. 2-color ELISPOT assay was performed. Digital image of a representative well is shown in panel A. Blue spots represent IFN-γ producing cells while red spots represent IL-2 producing cells. Spot forming units are represented in bar graph format in panel B.