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. Author manuscript; available in PMC: 2015 Jan 9.
Published in final edited form as: Vaccine. 2013 Nov 16;32(3):375–382. doi: 10.1016/j.vaccine.2013.11.023

Figure 4.

Figure 4

Functional C-terminal epitopes are masked by intra-molecular interactions. (A) Sera from mice immunized with full-length P1 (CG14), NA1, P3C, or a mixture of NA1 and P3C were used in a competition ELISA to evaluate inhibition of binding of biotin-labeled MAb 3–10E to S. mutans. Serum dilution is indicated on the x-axis. (B) BIAcore SPR analysis was used to evaluate bacterial adherence following incubation of S. mutans with sera collected from immunized mice. Values were normalized to the change in resonance units (ΔRU) for S. mutans NG8, in the absence of added serum, detected following the 60-second injection cycle. Immunogens are indicated on the X-axis. Tukey’s Multiple Comparison Test determined differences among the groups; * p≤0.05, **p≤0.01, ***p≤0.001, ****p≤0.0001. (C) The level of anti-S. mutans IgG in the sera from P1 versus truncated P1 -immunized mice was evaluated by serial dilution and standard ELISA. Data are representative of at least three independent experiments. Statistically significant differences compared to the CG14 group were determined by two-way ANOVA. p≤0.01 (P3C, NA1+P3C), p≤0.0001 (NA1).