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. 2013 Jun 10;2:138. [Version 1] doi: 10.12688/f1000research.2-138.v1

Figure 6. PCR analyses using primers specific to eight genes neighboring the CHLI1 locus.

Figure 6.

( A) PCR using the genomic DNA of 5A7 and 4A+ with primers specific to CHLI1 and four neighboring genes ( UP1, UP2, UP3, UP4) downstream of the CHLI1 gene. The sizes of the genomic DNA PCR products for CHLI1, UP1, UP2, UP3 and UP4 are, 459, 100, 342, 550 and 672 (bp), respectively. Odd numbered lanes denote 5A7; even numbered lanes denote 4A+; ST denotes 1 kb plus DNA ladder. ( B) PCR using the genomic DNA of 5A7 and 4A+ with primers specific to CHLI1 and four neighboring genes ( FDX3, AMT, UP5 and UP6) upstream of the CHLI1 gene. The sizes of the genomic DNA PCR products for CHLI1, FDX3, AMT, UP5 and UP6 are, 459, 90, 369, 379 and 369 (bp), respectively. Odd numbered lanes denote 5A7; even numbered lanes denote 4A+; M denotes 50 bp DNA ladder (NEB, Beverly, MA). ( C) PCR and RT-PCR with UP6 gene specific primers using the 5A7 and 4A+ genomic DNA and cDNA. Actin was used as a control. Actin genomic and cDNA product sizes are 527 and 305 (bp), respectively. Odd numbered lanes denote genomic DNA PCR products; even numbered lanes denote cDNA products. All primers used spanned an intron. M denotes 50 bp DNA ladder. All DNA samples were run on a 1.8% agarose gel. Gene names are given at the bottom of the gel. Primer sequences are shown in Table 2 and Table 3.