Table 4. List of primers for amplifying CHLI transcripts and complement testing.
Primer name | Sequence of primer | Gene/purpose |
---|---|---|
CHLI1CR | 5´-TTGACCCTTTGACACGAACCAACC-3´ | CHLI1 |
CHLI1BR | 5´-CATGCCGAACACCTGCTTGAAGAT-3´ | CHLI1 |
CHLI1AF | 5´-ACTACGACTTCCGCGTCAAGATCA-3´ | CHLI1 |
CHLI2BF | 5´-TGACGCATTTGTGGACTCGTGCAA-3´ | CHLI2 |
CHLI2CR | 5´-CACACTTACACGTTCACGCAGCAA-3´ | CHLI2 |
CHLI1XF | 5´-GGAATTCCATATGGCCTGAACATGCGTGTTTC-3´ | CHLI1 cDNA amplification for cloning |
CHLI1XR | 5´-CCGGAATTCTTACTCCATGCCGAACACCTGCTT-3´ | CHLI1 cDNA amplification for cloning |
PsaDF1 | 5´-CCACTGCTACTCACAACAAGCCCA-3´ | Complementation testing |