Skip to main content
. 2013 Jun 10;2:138. [Version 1] doi: 10.12688/f1000research.2-138.v1

Table 4. List of primers for amplifying CHLI transcripts and complement testing.

These primers were used in the experiments that generated the data in Figure 7 and Figure 10 and also used for CHLI1 cDNA amplification for cloning.

Primer name Sequence of primer Gene/purpose
CHLI1CR 5´-TTGACCCTTTGACACGAACCAACC-3´ CHLI1
CHLI1BR 5´-CATGCCGAACACCTGCTTGAAGAT-3´ CHLI1
CHLI1AF 5´-ACTACGACTTCCGCGTCAAGATCA-3´ CHLI1
CHLI2BF 5´-TGACGCATTTGTGGACTCGTGCAA-3´ CHLI2
CHLI2CR 5´-CACACTTACACGTTCACGCAGCAA-3´ CHLI2
CHLI1XF 5´-GGAATTCCATATGGCCTGAACATGCGTGTTTC-3´ CHLI1 cDNA amplification for cloning
CHLI1XR 5´-CCGGAATTCTTACTCCATGCCGAACACCTGCTT-3´ CHLI1 cDNA amplification for cloning
PsaDF1 5´-CCACTGCTACTCACAACAAGCCCA-3´ Complementation testing