Figure 4. The APHVIII flanking genomic DNA sequence in 5A7.

Primer 2R ( Table 1), specific to the 3´UTR of the APHVIII gene was used for sequencing the 850 bp Thermal Asymmetric InterLaced 2 (TAIL2) PCR product. 3´UTR sequence of APHVIII is in bold black, extra nucleotide additions are in bold blue. The flanking Chlamydomonas UP6 genomic sequence is denoted in red. The APHVIII end of the plasmid has been inserted after the eighth nucleotide in the fourth exon of UP6 gene.