Figure 8. A schematic figure of the pDBle vector used for complementation of chli1-1.

NdeI/ EcoR1 double digested CHLI1 cDNA (1260 bp) was cloned into the NdeI/ EcoRI double digested pDBle plasmid. Primers used for amplification of CHLI1 cDNA are shown in Table 4. CHLI1 expression is driven by the constitutive PsaD promoter. NdeI and EcoRI restriction sites are labeled. pDBle contains two copies of Ble ^R genes driven by the Rubisco ( RbcS2) promoter. The size of the CHLI1- pDBle construct is 7957 bp. Black arrow and white arrow denotes CHLI1 cDNA and Ble ^R gene, respectively. Grey boxes denote UnTranslated regions (UTR).