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. 1983 Oct;80(19):5842–5846. doi: 10.1073/pnas.80.19.5842

Cloning and expression of murine immune interferon cDNA.

P W Gray, D V Goeddel
PMCID: PMC390171  PMID: 6310596

Abstract

The murine immune interferon (IFN-gamma) gene was cloned and expressed under control of the simian virus 40 early promoter in the monkey COS-1 cell line. A protein is secreted from these cells having the biological, antigenic, and biochemical characteristics of natural murine IFN-gamma. Cloned murine IFN-gamma cDNAs were obtained by using RNA from both mitogen-induced murine spleens and the transfected COS cells, and both code for identical proteins. The mature murine IFN-gamma encoded is 136 amino acids long, 10 amino acids shorter than human IFN-gamma. The nucleotide homology between the murine and human IFN-gamma genes is 60-65%, whereas the encoded proteins are only 40% homologous. Murine IFN-gamma cDNA was expressed in Escherichia coli under trp promoter control.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benjamin W. R., Steeg P. S., Farrar J. J. Production of immune interferon by an interleukin 2-independent murine T cell line. Proc Natl Acad Sci U S A. 1982 Sep;79(17):5379–5383. doi: 10.1073/pnas.79.17.5379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berger S. L., Birkenmeier C. S. Inhibition of intractable nucleases with ribonucleoside--vanadyl complexes: isolation of messenger ribonucleic acid from resting lymphocytes. Biochemistry. 1979 Nov 13;18(23):5143–5149. doi: 10.1021/bi00590a018. [DOI] [PubMed] [Google Scholar]
  3. Crane J. L., Jr, Glasgow L. A., Kern E. R., Youngner J. S. Inhibition of murine osteogenic sarcomas by treatment with type I or type II interferon. J Natl Cancer Inst. 1978 Sep;61(3):871–874. [PubMed] [Google Scholar]
  4. Crowley C. W., Liu C. C., Levinson A. D. Plasmid-directed synthesis of hepatitis B surface antigen in monkey cells. Mol Cell Biol. 1983 Jan;3(1):44–55. doi: 10.1128/mcb.3.1.44. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Degrave W., Derynck R., Tavernier J., Haegeman G., Fiers W. Nucleotide sequence of the chromosomal gene for human fibroblast (beta 1) interferon and of the flanking regions. Gene. 1981 Aug;14(3):137–143. doi: 10.1016/0378-1119(81)90109-8. [DOI] [PubMed] [Google Scholar]
  6. Gluzman Y. SV40-transformed simian cells support the replication of early SV40 mutants. Cell. 1981 Jan;23(1):175–182. doi: 10.1016/0092-8674(81)90282-8. [DOI] [PubMed] [Google Scholar]
  7. Goeddel D. V., Leung D. W., Dull T. J., Gross M., Lawn R. M., McCandliss R., Seeburg P. H., Ullrich A., Yelverton E., Gray P. W. The structure of eight distinct cloned human leukocyte interferon cDNAs. Nature. 1981 Mar 5;290(5801):20–26. doi: 10.1038/290020a0. [DOI] [PubMed] [Google Scholar]
  8. Goeddel D. V., Shepard H. M., Yelverton E., Leung D., Crea R., Sloma A., Pestka S. Synthesis of human fibroblast interferon by E. coli. Nucleic Acids Res. 1980 Sep 25;8(18):4057–4074. doi: 10.1093/nar/8.18.4057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Goeddel D. V., Yelverton E., Ullrich A., Heyneker H. L., Miozzari G., Holmes W., Seeburg P. H., Dull T., May L., Stebbing N. Human leukocyte interferon produced by E. coli is biologically active. Nature. 1980 Oct 2;287(5781):411–416. doi: 10.1038/287411a0. [DOI] [PubMed] [Google Scholar]
  10. Gray P. W., Goeddel D. V. Structure of the human immune interferon gene. Nature. 1982 Aug 26;298(5877):859–863. doi: 10.1038/298859a0. [DOI] [PubMed] [Google Scholar]
  11. Gray P. W., Leung D. W., Pennica D., Yelverton E., Najarian R., Simonsen C. C., Derynck R., Sherwood P. J., Wallace D. M., Berger S. L. Expression of human immune interferon cDNA in E. coli and monkey cells. Nature. 1982 Feb 11;295(5849):503–508. doi: 10.1038/295503a0. [DOI] [PubMed] [Google Scholar]
  12. Havell E. A., Spitalny G. L., Patel P. J. Enhanced production of murine interferon gamma by T cells generated in response to bacterial infection. J Exp Med. 1982 Jul 1;156(1):112–127. doi: 10.1084/jem.156.1.112. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Heremans H., De Ley M., Billiau A., De Somer P. Interferon induced in mouse spleen cells by Staphylococcus aureus. Cell Immunol. 1982 Aug;71(2):353–364. doi: 10.1016/0008-8749(82)90269-6. [DOI] [PubMed] [Google Scholar]
  14. Langford M. P., Georgiades J. A., Stanton G. J., Dianzani F., Johnson H. M. Large-scale production and physicochemical characterization of human immune interferon. Infect Immun. 1979 Oct;26(1):36–41. doi: 10.1128/iai.26.1.36-41.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lawn R. M., Adelman J., Franke A. E., Houck C. M., Gross M., Najarian R., Goeddel D. V. Human fibroblast interferon gene lacks introns. Nucleic Acids Res. 1981 Mar 11;9(5):1045–1052. doi: 10.1093/nar/9.5.1045. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lawn R. M., Fritsch E. F., Parker R. C., Blake G., Maniatis T. The isolation and characterization of linked delta- and beta-globin genes from a cloned library of human DNA. Cell. 1978 Dec;15(4):1157–1174. doi: 10.1016/0092-8674(78)90043-0. [DOI] [PubMed] [Google Scholar]
  17. Lawn R. M., Gross M., Houck C. M., Franke A. E., Gray P. V., Goeddel D. V. DNA sequence of a major human leukocyte interferon gene. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5435–5439. doi: 10.1073/pnas.78.9.5435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lusky M., Botchan M. Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequences. Nature. 1981 Sep 3;293(5827):79–81. doi: 10.1038/293079a0. [DOI] [PubMed] [Google Scholar]
  19. Maniatis T., Hardison R. C., Lacy E., Lauer J., O'Connell C., Quon D., Sim G. K., Efstratiadis A. The isolation of structural genes from libraries of eucaryotic DNA. Cell. 1978 Oct;15(2):687–701. doi: 10.1016/0092-8674(78)90036-3. [DOI] [PubMed] [Google Scholar]
  20. Marcucci F., Nowak M., Krammer P., Kirchner H. Production of high titres of interferon-gamma by cells derived from short-term cultures of murine spleen leukocytes in T-cell growth factor-conditioned medium. J Gen Virol. 1982 May;60(Pt 1):195–198. doi: 10.1099/0022-1317-60-1-195. [DOI] [PubMed] [Google Scholar]
  21. Marcucci F., Waller M., Kirchner H., Krammer P. Production of immune interferon by murine T-cell clones from long-term cultures. Nature. 1981 May 7;291(5810):79–81. doi: 10.1038/291079a0. [DOI] [PubMed] [Google Scholar]
  22. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  23. Myers R. M., Tjian R. Construction and analysis of simian virus 40 origins defective in tumor antigen binding and DNA replication. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6491–6495. doi: 10.1073/pnas.77.11.6491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Nagata S., Mantei N., Weissmann C. The structure of one of the eight or more distinct chromosomal genes for human interferon-alpha. Nature. 1980 Oct 2;287(5781):401–408. doi: 10.1038/287401a0. [DOI] [PubMed] [Google Scholar]
  25. Naylor S. L., Sakaguchi A. Y., Shows T. B., Law M. L., Goeddel D. V., Gray P. W. Human immune interferon gene is located on chromosome 12. J Exp Med. 1983 Mar 1;157(3):1020–1027. doi: 10.1084/jem.157.3.1020. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Ohno S., Taniguchi T. Structure of a chromosomal gene for human interferon beta. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5305–5309. doi: 10.1073/pnas.78.9.5305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Osborne L. C., Georgiades J. A., Johnson H. M. Large-scale production and partial purification of mouse immune interferon. Infect Immun. 1979 Jan;23(1):80–86. doi: 10.1128/iai.23.1.80-86.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Owerbach D., Rutter W. J., Shows T. B., Gray P., Goeddel D. V., Lawn R. M. Leukocyte and fibroblast interferon genes are located on human chromosome 9. Proc Natl Acad Sci U S A. 1981 May;78(5):3123–3127. doi: 10.1073/pnas.78.5.3123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Shaw G. D., Boll W., Taira H., Mantei N., Lengyel P., Weissmann C. Structure and expression of cloned murine IFN-alpha genes. Nucleic Acids Res. 1983 Feb 11;11(3):555–573. doi: 10.1093/nar/11.3.555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Smith A. J. DNA sequence analysis by primed synthesis. Methods Enzymol. 1980;65(1):560–580. doi: 10.1016/s0076-6879(80)65060-5. [DOI] [PubMed] [Google Scholar]
  31. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  32. Taylor J. M., Illmensee R., Summers J. Efficeint transcription of RNA into DNA by avian sarcoma virus polymerase. Biochim Biophys Acta. 1976 Sep 6;442(3):324–330. doi: 10.1016/0005-2787(76)90307-5. [DOI] [PubMed] [Google Scholar]
  33. Weck P. K., Apperson S., Stebbing N., Gray P. W., Leung D., Shepard H. M., Goeddel D. V. Antiviral activities of hybrids of two major human leukocyte interferons. Nucleic Acids Res. 1981 Nov 25;9(22):6153–6166. doi: 10.1093/nar/9.22.6153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Wietzerbin J., Stefanos S., Lucero M., Falcoff E., O'Malley J. A., Sulkowski E. Physico-chemical characterization and partial purification of mouse immune interferon. J Gen Virol. 1979 Sep;44(3):773–781. doi: 10.1099/0022-1317-44-3-773. [DOI] [PubMed] [Google Scholar]
  35. Wong G. H., Clark-Lewis I., McKimm-Breschkin J. L., Schrader J. W. Interferon-gamma-like molecule induces Ia antigens on cultured mast cell progenitors. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6989–6993. doi: 10.1073/pnas.79.22.6989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Yip Y. K., Barrowclough B. S., Urban C., Vilcek J. Molecular weight of human gamma interferon is similar to that of other human interferons. Science. 1982 Jan 22;215(4531):411–413. doi: 10.1126/science.6173921. [DOI] [PubMed] [Google Scholar]

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