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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1983 Oct;80(19):5847–5851. doi: 10.1073/pnas.80.19.5847

13C NMR studies of acetate metabolism during sporulation of Saccharomyces cerevisiae.

J R Dickinson, I W Dawes, A S Boyd, R L Baxter
PMCID: PMC390172  PMID: 6136970

Abstract

The sporulation of Saccharomyces cerevisiae in the presence of [2-13C]acetate was studied by 13C NMR spectroscopy. The fate of 13C label was analyzed in vivo and in cell extracts. During the first 4 hr of sporulation the major metabolite produced from [2-13C]acetate utilization was glutamate. From the labeling pattern observed it is concluded that both the tricarboxylic acid cycle and the glyoxylate cycle are operating. After about 4 hr trehalose is made. Comparison of the doublet/singlet ratios for C-1,1(1) and C-6,6(1) of trehalose shows a steady drop in the ratio of C-1, C-2-coupled species over trehalose labeled only at C-1 in the C-1, 2 segment of the molecule. The negative correlation of this ratio with that for the C-5, 6 segment indicates a cycling of glucose through the hexose monophosphate shunt. Subsequently fatty acid biosynthesis commences. Large amounts of saturated fatty acid were made. There were conspicuous differences observed in the metabolism of [2-13C]acetate between sporulating and vegetatively growing cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dawes I. W. Study of cell development using depressed mutations. Nature. 1975 Jun 26;255(5511):707–708. doi: 10.1038/255707a0. [DOI] [PubMed] [Google Scholar]
  2. Esposito M. S., Esposito R. E., Arnaud M., Halvorson H. O. Acetate utilization and macromolecular synthesis during sporulation of yeast. J Bacteriol. 1969 Oct;100(1):180–186. doi: 10.1128/jb.100.1.180-186.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fast D. Sporulation synchrony of Saccharomyces cerevisiae grown in various carbon sources. J Bacteriol. 1973 Nov;116(2):925–930. doi: 10.1128/jb.116.2.925-930.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Illingworth R. F., Rose A. H., Beckett A. Changes in the lipid composition and fine structure of Saccharomyces cerevisiae during ascus formation. J Bacteriol. 1973 Jan;113(1):373–386. doi: 10.1128/jb.113.1.373-386.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Miyake S., Sando N., Sato S. Biochemical changes in yeast during sporulation. II. Acetate metabolism. Dev Growth Differ. 1971 Feb;12(4):285–296. doi: 10.1111/j.1440-169x.1971.285_n.x. [DOI] [PubMed] [Google Scholar]
  6. RAMIREZ C., MILLER J. J. THE METABOLISM OF YEAST SPORULATION. VI. CHANGES IN AMINO ACID CONTENT DURING SPOROGENESIS. Can J Microbiol. 1964 Oct;10:623–631. doi: 10.1139/m64-082. [DOI] [PubMed] [Google Scholar]
  7. Walker T. E., Han C. H., Kollman V. H., London R. E., Matwiyoff N. A. 13C nuclear magnetic resonance studies of the biosynthesis by Microbacterium ammoniaphilum of L-glutamate selectively enriched with carbon-13. J Biol Chem. 1982 Feb 10;257(3):1189–1195. [PubMed] [Google Scholar]
  8. Wright J. F., Ajam N., Dawes I. W. Nature and timing of some sporulation-specific protein changes in Saccharomyces cerevisiae. Mol Cell Biol. 1981 Oct;1(10):910–918. doi: 10.1128/mcb.1.10.910. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. den Hollander J. A., Behar K. L., Shulman R. G. 13C NMR study of transamination during acetate utilization by Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1981 May;78(5):2693–2697. doi: 10.1073/pnas.78.5.2693. [DOI] [PMC free article] [PubMed] [Google Scholar]

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