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. 1983 Oct;80(19):5890–5894. doi: 10.1073/pnas.80.19.5890

Z-DNA immunoreactivity in fixed metaphase chromosomes of primates.

E Viegas-Péquignot, C Derbin, B Malfoy, E Taillandier, M Leng, B Dutrillaux
PMCID: PMC390181  PMID: 6351068

Abstract

Antibodies against Z-DNA bind to fixed metaphase chromosomes of man and Cebus albifrons (Platyrrhini, Primate). By indirect immunofluorescence and indirect immunoperoxidase techniques, a heavy staining is detected in some segments of chromosomes of C. albifrons. These segments correspond to R-band-positive heterochromatin, which has a high G + C-base content. Euchromatin of human and Cebus chromosomes show a weak and heterogeneous staining that consistently reproduces an R- and T-banding pattern in both species. Because chromosome homologies previously were demonstrated between these distantly related species by chromosome banding, our results suggest that Z-DNA has been conserved during the course of primate evolution.

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