Fig. 7.
Bioluminescence imaging of CAST mice infected with Wyeth-vFire. Three female CAST mice were infected intranasally with 107 PFU of Wyeth-vFire. (A) Representative images of the heads and ventral torsos. The day post-infection on which images were obtained is indicated above each image. The yellow boxes outline the ROI used to calculate photon flux. Bioluminescent images of the head were obtained using an f/stop of 1, binning factor of 4, and an acquisition time of 1 s. The same color scale set between 600 and 60,000 was used for all three panels of pictures. Images of the ventral torso were acquired using an f/stop of 1, binning factor of 8, and an acquisition time of 60 s. (B) ROI analysis was performed on the head, chest, and abdomen of infected mice to calculate total photon flux. Data represent mean group values for photon flux. Bars represent SEM.