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. 1983 Nov;80(21):6528–6532. doi: 10.1073/pnas.80.21.6528

Genomic cloning and preliminary characterization of the human thymidine kinase gene.

P F Lin, S Y Zhao, F H Ruddle
PMCID: PMC390386  PMID: 6314329

Abstract

In this report, we describe the cloning of the human cytoplasmic thymidine kinase (tk-C; EC 2.7.1.21) gene and its preliminary characterization. The tk-C sequences were isolated from a phage genomic library made from DNA of a transfected mouse cell carrying the human tk-C gene. The human transforming sequences were identified by homology with human Alu sequences. Six recombinant phages were isolated and five were competent to transfer human TK-C activity to TK-deficient mouse cells when transferred in pairs. Conclusively, sequences homologous to these clones are present in all human TK+ transformants examined. We estimate the maximal size of the tk-C gene to be 14 kilobase pairs and its minimal size to be between 4 and 5 kilobase pairs. The gene contains many noncoding inserts and numerous Alu sequences.

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Selected References

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