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. 1985 Jul;82(13):4374–4378. doi: 10.1073/pnas.82.13.4374

Cloning, characterization, and sequence of the yeast DNA topoisomerase I gene.

C Thrash, A T Bankier, B G Barrell, R Sternglanz
PMCID: PMC390416  PMID: 2989818

Abstract

The structural gene for yeast DNA topoisomerase I (TOP1) has been cloned from two yeast genomic plasmid banks. Integration of a plasmid carrying the gene into the chromosome and subsequent genetic mapping shows that TOP1 is identical to the gene previously called MAK1. Seven top1 (mak1) mutants including gene disruptions are viable, demonstrating that DNA topoisomerase I is not essential for viability in yeast. A 3787-base-pair DNA fragment including the gene has been sequenced. The protein predicted from the DNA sequence has 769 amino acids and a molecular weight of 90,020.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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