Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1985 Aug;82(15):5180–5184. doi: 10.1073/pnas.82.15.5180

Duck hepatitis B virus DNA in liver and serum of Chinese ducks: integration of viral DNA in a hepatocellular carcinoma.

O Yokosuka, M Omata, Y Z Zhou, F Imazeki, K Okuda
PMCID: PMC390523  PMID: 3860852

Abstract

The presence of duck hepatitis B virus (DHBV) DNA in liver and serum and its state (integrated vs. free) were studied in 23 ducks from Chi-tung county in China by spot hybridization and Southern blot hybridization, respectively. In 16 of 23 (70%), DHBV DNA was detected in serum and/or in liver tissue. These infected ducks showed a variety of pathological changes including advanced chronic disease in the liver. In contrast, none of the virus-negative ducks had advanced hepatic changes. One DHBV DNA-seropositive duck had a large hepatocellular carcinoma. Southern blot analysis demonstrated integrated DHBV DNA in neoplastic tissue and abundant episomal DHBV DNA in non-neoplastic tissue of the liver. In one noninfected duck with a small adenoma, no viral DNA was detected in tumor or non-neoplastic tissue. The detection of integrated DHBV DNA in hepatocellular carcinoma suggests that DHBV behaves like human and woodchuck hepatitis viruses in relation to chronic liver disease and hepatocarcinogenesis.

Full text

PDF
5180

Images in this article

5181Image
on p.5181
5181Image
on p.5181
5183Image
on p.5183
5183Image
on p.5183
5183Image
on p.5183
5183Image
on p.5183
5184Image
on p.5184

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brechot C., Pourcel C., Louise A., Rain B., Tiollais P. Presence of integrated hepatitis B virus DNA sequences in cellular DNA of human hepatocellular carcinoma. Nature. 1980 Jul 31;286(5772):533–535. doi: 10.1038/286533a0. [DOI] [PubMed] [Google Scholar]
  2. Chakraborty P. R., Ruiz-Opazo N., Shouval D., Shafritz D. A. Identification of integrated hepatitis B virus DNA and expression of viral RNA in an HBsAg-producing human hepatocellular carcinoma cell line. Nature. 1980 Jul 31;286(5772):531–533. doi: 10.1038/286531a0. [DOI] [PubMed] [Google Scholar]
  3. Edman J. C., Gray P., Valenzuela P., Rall L. B., Rutter W. J. Integration of hepatitis B virus sequences and their expression in a human hepatoma cell. Nature. 1980 Jul 31;286(5772):535–538. doi: 10.1038/286535a0. [DOI] [PubMed] [Google Scholar]
  4. Halpern M. S., England J. M., Deery D. T., Petcu D. J., Mason W. S., Molnar-Kimber K. L. Viral nucleic acid synthesis and antigen accumulation in pancreas and kidney of Pekin ducks infected with duck hepatitis B virus. Proc Natl Acad Sci U S A. 1983 Aug;80(15):4865–4869. doi: 10.1073/pnas.80.15.4865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Marion P. L., Knight S. S., Ho B. K., Guo Y. Y., Robinson W. S., Popper H. Liver disease associated with duck hepatitis B virus infection of domestic ducks. Proc Natl Acad Sci U S A. 1984 Feb;81(3):898–902. doi: 10.1073/pnas.81.3.898. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Marion P. L., Oshiro L. S., Regnery D. C., Scullard G. H., Robinson W. S. A virus in Beechey ground squirrels that is related to hepatitis B virus of humans. Proc Natl Acad Sci U S A. 1980 May;77(5):2941–2945. doi: 10.1073/pnas.77.5.2941. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Marion P. L., Salazar F. H., Alexander J. J., Robinson W. S. State of hepatitis B viral DNA in a human hepatoma cell line. J Virol. 1980 Feb;33(2):795–806. doi: 10.1128/jvi.33.2.795-806.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mason W. S., Halpern M. S., England J. M., Seal G., Egan J., Coates L., Aldrich C., Summers J. Experimental transmission of duck hepatitis B virus. Virology. 1983 Dec;131(2):375–384. doi: 10.1016/0042-6822(83)90505-6. [DOI] [PubMed] [Google Scholar]
  9. Mason W. S., Seal G., Summers J. Virus of Pekin ducks with structural and biological relatedness to human hepatitis B virus. J Virol. 1980 Dec;36(3):829–836. doi: 10.1128/jvi.36.3.829-836.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Omata M., Uchiumi K., Ito Y., Yokosuka O., Mori J., Terao K., Wei-Fa Y., O'Connell A. P., London W. T., Okuda K. Duck hepatitis B virus and liver diseases. Gastroenterology. 1983 Aug;85(2):260–267. [PubMed] [Google Scholar]
  11. Omata M., Yokosuka O., Imazeki F., Matsuyama Y., Uchiumi K., Ito Y., Mori J., Okuda K. Transmission of duck hepatitis B virus from Chinese carrier ducks to Japanese ducklings: a study of viral DNA in serum and tissue. Hepatology. 1984 Jul-Aug;4(4):603–607. doi: 10.1002/hep.1840040404. [DOI] [PubMed] [Google Scholar]
  12. Patterson M., Gonzalez-Vitale J. C., Fagan C. J. Polycystic liver disease: a study of cyst fluid constituents. Hepatology. 1982 Jul-Aug;2(4):475–478. doi: 10.1002/hep.1840020414. [DOI] [PubMed] [Google Scholar]
  13. Shapiro L. E., Samuels H. H., Yaffe B. M. Thyroid and glucocorticoid hormones synergistically control growth hormone mRNA in cultured GH1 cells. Proc Natl Acad Sci U S A. 1978 Jan;75(1):45–49. doi: 10.1073/pnas.75.1.45. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  15. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES