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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1985 Aug;82(15):5213–5217. doi: 10.1073/pnas.82.15.5213

Glycyl-L-glutamine, a precursor, and glycyl-L-glutamic acid, a neurotrophic factor for maintenance of acetylcholinesterase and butyrylcholinesterase in the preganglionically denervated superior cervical ganglion of the cat in vivo.

G B Koelle, U J Sanville, S J Wall
PMCID: PMC390530  PMID: 3860856

Abstract

L. W. Haynes and M. E. Smith have reported [(1985) Biochem. Soc. Trans. 13, 174-175] that glycyl-L-glutamine (Gly-Gln) increases the A12 and G4 forms of acetylcholinesterase (AcChoEase) in cultured embryonic rat skeletal muscle. Since Gly-Gln meets the criteria established for the neurotrophic factor (NF) in extracts of central nervous system/sciatic nerves that maintains AcChoEase and butyrylcholinesterase (BtChoEase) in the denervated cat superior cervical ganglion (SCG) in vivo, it was tested by the latter procedure. Solutions of Gly-Gln (10(-7)-10(-3) M) in 0.9% NaCl solution were infused for 24 hr via the right common carotid artery of cats with preganglionically denervated SCG, following ligation of the external carotid and lingual arteries. At 48 hr postdenervation, the AcChoEase and BtChoEase contents of the right SCG were within the range of similarly treated controls infused with 0.9% NaCl solution; the AcChoEase and BtChoEase contents of the left SCG, where the infused solutions arrived by way of a much more circuitous route, were significantly elevated at concentrations of Gly-Gln of 10(-5) M and higher. This suggested that the neurotrophic effect on the left SCG was produced by a metabolite of Gly-Gln. Accordingly, glycine, L-glutamine, and glycyl-L-glutamic acid (Gly-Glu) were then tested. Glycine and L-glutamine were inactive; Gly-Glu, 10(-6)-10(-5) M, exerted a significantly positive neurotrophic effect at both the right and left SCG; at 10(-4) M, the effect was absent. The method employed currently for preparation of extracts of SCG for assay of AcChoEase, BtChoEase, and protein contents (homogenization of scissor-minced ganglia in water) was compared with homogenization in molar NaCl/1% Triton X-100. Values obtained by the former procedure, in comparison with the latter, were 91% +/- 7% for AcChoEase and 83% +/- 7% for BtChoEase, expressed as substrate hydrolyzed per mg of protein per min.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Davis R., Koelle G. B. Electron microscope localization of acetylcholinesterase and butyrylcholinesterase in the superior cervical ganglion of the cat. I. Normal ganglion. J Cell Biol. 1978 Sep;78(3):785–809. doi: 10.1083/jcb.78.3.785. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Davis R., Koelle G. B. Electron microscope localization of acetylcholinesterase and butyrylcholinesterase in the superior cervical ganglion of the cat. II. Preganglionically denervated ganglion. J Cell Biol. 1981 Mar;88(3):581–590. doi: 10.1083/jcb.88.3.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. ELLMAN G. L., COURTNEY K. D., ANDRES V., Jr, FEATHER-STONE R. M. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol. 1961 Jul;7:88–95. doi: 10.1016/0006-2952(61)90145-9. [DOI] [PubMed] [Google Scholar]
  4. Haynes L. W., Smith M. E., Smyth D. G. Evidence for the neurotrophic regulation of collagen-tailed acetylcholinesterase in immature skeletal muscle by beta-endorphin. J Neurochem. 1984 Jun;42(6):1542–1551. doi: 10.1111/j.1471-4159.1984.tb12740.x. [DOI] [PubMed] [Google Scholar]
  5. Koelle G. B., Davis R., Koelle W. A. Effects of aldehyde fixation and of preganglionic denervation on acetylcholinesterase and butyrocholinesterase of cat autonomic ganglia. J Histochem Cytochem. 1974 Apr;22(4):244–251. doi: 10.1177/22.4.244. [DOI] [PubMed] [Google Scholar]
  6. Koelle G. B., Ruch G. A. Demonstration of a neurotrophic factor for the maintenance of acetylcholinesterase and butyrylcholinesterase in the preganglionically denervated superior cervical ganglion of the cat. Proc Natl Acad Sci U S A. 1983 May;80(10):3106–3110. doi: 10.1073/pnas.80.10.3106. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Koelle G. B., Ruch G. A., Rickard K. K., Sanville U. J. Regeneration of cholinesterases in the stellate and normal and denervated superior cervical ganglion of the cat following inactivation by sarin. J Neurochem. 1982 Jun;38(6):1695–1698. doi: 10.1111/j.1471-4159.1982.tb06651.x. [DOI] [PubMed] [Google Scholar]
  8. Koelle G. B., Ruch G. A., Uchida E. Effects of sodium pentobarbital anesthesia and neurotrophic factor on the maintenance of acetylcholinesterase and butyrylcholinesterase in the preganglionically denervated superior cervical ganglion of the cat. Proc Natl Acad Sci U S A. 1983 Oct;80(19):6122–6125. doi: 10.1073/pnas.80.19.6122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Koelle G. B., Sanville U. J., Rickard K. K., Williams J. E. Partial characterization of the neurotrophic factor for maintenance of acetylcholinesterase and butyrylcholinesterase in the preganglionically denervated superior cervical ganglion of the cat in vivo. Proc Natl Acad Sci U S A. 1984 Oct;81(20):6539–6542. doi: 10.1073/pnas.81.20.6539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Massoulié J., Bon S. The molecular forms of cholinesterase and acetylcholinesterase in vertebrates. Annu Rev Neurosci. 1982;5:57–106. doi: 10.1146/annurev.ne.05.030182.000421. [DOI] [PubMed] [Google Scholar]
  12. Parish D. C., Smyth D. G., Normanton J. R., Wolstencroft J. H. Glycyl glutamine, an inhibitory neuropeptide derived from beta-endorphin. Nature. 1983 Nov 17;306(5940):267–270. doi: 10.1038/306267a0. [DOI] [PubMed] [Google Scholar]

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