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. 1985 Aug;82(15):5231–5235. doi: 10.1073/pnas.82.15.5231

Inositol trisphosphate-induced calcium release and contraction in vascular smooth muscle.

A V Somlyo, M Bond, A P Somlyo, A Scarpa
PMCID: PMC390534  PMID: 2991913

Abstract

Inositol 1,4,5-trisphosphate (InsP3) caused Ca release and tension development in rabbit main pulmonary artery smooth muscle permeabilized with saponin or digitonin. Both of these responses to single additions of InsP3 (0.5-30 microM) were repeatable and occurred in the presence of 0.0-1.9 mM free Mg2+. Sustained contractions were induced by InsP3. The amount of Ca released by InsP3, measured with a Ca2+-selective electrode, was also estimated to be sufficient to stimulate contraction in intact smooth muscle. Ca release was not influenced by inhibitors of mitochondrial oxidative phosphorylation. The uptake of Ca2+ from the medium into the InsP3-sensitive pool was ATP-dependent. The present results support the hypothesis that, in smooth muscle, InsP3 is the messenger, or one of the messengers, involved in transmitter-induced (pharmacomechanical) Ca release from the sarcoplasmic reticulum, which is the intracellular Ca store identified previously as the source of Ca released by norepinephrine in main pulmonary artery.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akhtar R. A., Abdel-Latif A. A. Carbachol causes rapid phosphodiesteratic cleavage of phosphatidylinositol 4,5-bisphosphate and accumulation of inositol phosphates in rabbit iris smooth muscle; prazosin inhibits noradrenaline- and ionophore A23187-stimulated accumulation of inositol phosphates. Biochem J. 1984 Nov 15;224(1):291–300. doi: 10.1042/bj2240291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baron C. B., Cunningham M., Strauss J. F., 3rd, Coburn R. F. Pharmacomechanical coupling in smooth muscle may involve phosphatidylinositol metabolism. Proc Natl Acad Sci U S A. 1984 Nov;81(21):6899–6903. doi: 10.1073/pnas.81.21.6899. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berridge M. J., Irvine R. F. Inositol trisphosphate, a novel second messenger in cellular signal transduction. Nature. 1984 Nov 22;312(5992):315–321. doi: 10.1038/312315a0. [DOI] [PubMed] [Google Scholar]
  4. Bond M., Kitazawa T., Somlyo A. P., Somlyo A. V. Release and recycling of calcium by the sarcoplasmic reticulum in guinea-pig portal vein smooth muscle. J Physiol. 1984 Oct;355:677–695. doi: 10.1113/jphysiol.1984.sp015445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Casteels R. Electro- and pharmacomechanical coupling in vascular smooth muscle. Chest. 1980 Jul;78(1 Suppl):150–156. doi: 10.1378/chest.78.1_supplement.150. [DOI] [PubMed] [Google Scholar]
  6. Coburn R. F., Yamaguchi T. Membrane potential-dependent and-independent tension in the canine tracheal muscle. J Pharmacol Exp Ther. 1977 May;201(2):276–284. [PubMed] [Google Scholar]
  7. Devine C. E., Somlyo A. V., Somlyo A. P. Sarcoplasmic reticulum and excitation-contraction coupling in mammalian smooth muscles. J Cell Biol. 1972 Mar;52(3):690–718. doi: 10.1083/jcb.52.3.690. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Endo M., Yagi S., Iino M. Tension-pCa relation and sarcoplasmic reticulum responses in chemically skinned smooth muscle fibers. Fed Proc. 1982 May;41(7):2245–2250. [PubMed] [Google Scholar]
  9. Fabiato A., Fabiato F. Calculator programs for computing the composition of the solutions containing multiple metals and ligands used for experiments in skinned muscle cells. J Physiol (Paris) 1979;75(5):463–505. [PubMed] [Google Scholar]
  10. Fiskum G., Craig S. W., Decker G. L., Lehninger A. L. The cytoskeleton of digitonin-treated rat hepatocytes. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3430–3434. doi: 10.1073/pnas.77.6.3430. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gomperts B. D. Involvement of guanine nucleotide-binding protein in the gating of Ca2+ by receptors. Nature. 1983 Nov 3;306(5938):64–66. doi: 10.1038/306064a0. [DOI] [PubMed] [Google Scholar]
  12. HOKIN M. R., HOKIN L. E. Enzyme secretion and the incorporation of P32 into phospholipides of pancreas slices. J Biol Chem. 1953 Aug;203(2):967–977. [PubMed] [Google Scholar]
  13. Irvine R. F., Letcher A. J., Lander D. J., Downes C. P. Inositol trisphosphates in carbachol-stimulated rat parotid glands. Biochem J. 1984 Oct 1;223(1):237–243. doi: 10.1042/bj2230237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Itoh T., Kuriyama H., Suzuki H. Differences and similarities in the noradrenaline- and caffeine-induced mechanical responses in the rabbit mesenteric artery. J Physiol. 1983 Apr;337:609–629. doi: 10.1113/jphysiol.1983.sp014645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Jones L. M., Michell R. H. The relationship of calcium to receptor-controlled stimulation of phosphatidylinositol turnover. Effects of acetylcholine, adrenaline, calcium ions, cinchocaine and a bivalent cation ionophore on rat parotid-gland fragments. Biochem J. 1975 Jun;148(3):479–485. doi: 10.1042/bj1480479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lapetina E. G., Briley P. A., De Robertis E. Effect of adrenergic agonists on phosphatidylinositol labelling in heart and aorta. Biochim Biophys Acta. 1976 Jun 22;431(3):624–630. doi: 10.1016/0005-2760(76)90226-5. [DOI] [PubMed] [Google Scholar]
  17. Leijten P. A., van Breemen C. The effects of caffeine on the noradrenaline-sensitive calcium store in rabbit aorta. J Physiol. 1984 Dec;357:327–339. doi: 10.1113/jphysiol.1984.sp015502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Martonosi A. N. Mechanisms of Ca2+ release from sarcoplasmic reticulum of skeletal muscle. Physiol Rev. 1984 Oct;64(4):1240–1320. doi: 10.1152/physrev.1984.64.4.1240. [DOI] [PubMed] [Google Scholar]
  19. Prentki M., Wollheim C. B., Lew P. D. Ca2+ homeostasis in permeabilized human neutrophils. Characterization of Ca2+-sequestering pools and the action of inositol 1,4,5-triphosphate. J Biol Chem. 1984 Nov 25;259(22):13777–13782. [PubMed] [Google Scholar]
  20. Somlyo A. P. Cell physiology: cellular site of calcium regulation. Nature. 1984 Jun 7;309(5968):516–517. doi: 10.1038/309516b0. [DOI] [PubMed] [Google Scholar]
  21. Somlyo A. P., Devine C. E., Somlyo A. V., North S. R. Sarcoplasmic reticulum and the temperature-dependent contraction of smooth muscle in calcium-free solutions. J Cell Biol. 1971 Dec;51(3):722–741. doi: 10.1083/jcb.51.3.722. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Somlyo A. P., Somlyo A. V., Shuman H., Endo M. Calcium and monovalent ions in smooth muscle. Fed Proc. 1982 Oct;41(12):2883–2890. [PubMed] [Google Scholar]
  23. Somlyo A. V., Somlyo A. P. Electromechanical and pharmacomechanical coupling in vascular smooth muscle. J Pharmacol Exp Ther. 1968 Jan;159(1):129–145. [PubMed] [Google Scholar]
  24. Somlyo A. V., Vinall P., Somlyo A. P. Excitation-contraction coupling and electrical events in two types of vascular smooth muscle. Microvasc Res. 1969 Oct;1(4):354–373. doi: 10.1016/0026-2862(69)90014-4. [DOI] [PubMed] [Google Scholar]
  25. Streb H., Irvine R. F., Berridge M. J., Schulz I. Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphate. Nature. 1983 Nov 3;306(5938):67–69. doi: 10.1038/306067a0. [DOI] [PubMed] [Google Scholar]
  26. Suematsu E., Hirata M., Hashimoto T., Kuriyama H. Inositol 1,4,5-trisphosphate releases Ca2+ from intracellular store sites in skinned single cells of porcine coronary artery. Biochem Biophys Res Commun. 1984 Apr 30;120(2):481–485. doi: 10.1016/0006-291x(84)91279-8. [DOI] [PubMed] [Google Scholar]
  27. Takenawa T. Inositol phospholipids in stimulated smooth muscles. Cell Calcium. 1982 Oct;3(4-5):359–368. doi: 10.1016/0143-4160(82)90023-9. [DOI] [PubMed] [Google Scholar]
  28. Wuytack F., Casteels R. Demonstration of a (Ca2+ + Mg2+)-ATPase activity probably related to Ca2+ transport in the microsomal fraction of porcine coronary artery smooth muscle. Biochim Biophys Acta. 1980 Jan 25;595(2):257–263. doi: 10.1016/0005-2736(80)90088-7. [DOI] [PubMed] [Google Scholar]

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