Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1985 Aug;82(16):5270–5274. doi: 10.1073/pnas.82.16.5270

Assignment of the gene for human DNA polymerase alpha to the X chromosome.

T S Wang, B E Pearson, H A Suomalainen, T Mohandas, L J Shapiro, J Schröder, D Korn
PMCID: PMC390549  PMID: 2410918

Abstract

We have applied an assay based on a monoclonal antibody that discriminates the activity of human DNA polymerase alpha in rodent-human somatic cell hybrid clones to identify a single genetic locus that is both necessary and sufficient for the expression of DNA polymerase alpha. We have mapped this locus to the short arm of the human X chromosome, near the junction of bands Xp21.3 and Xp22.1, and demonstrated that it is not expressed from an inactive X chromosome.

Full text

PDF
5270

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnold W. J., Kelley W. N. Human hypoxanthine-guanine phosphoribosyltransferase. Purification and subunit structure. J Biol Chem. 1971 Dec 10;246(23):7398–7404. [PubMed] [Google Scholar]
  2. Duncan C. H., Jagadeeswaran P., Wang R. R., Weissman S. M. Structural analysis of templates and RNA polymerase III transcripts of Alu family sequences interspersed among the human beta-like globin genes. Gene. 1981 Mar;13(2):185–196. doi: 10.1016/0378-1119(81)90007-x. [DOI] [PubMed] [Google Scholar]
  3. Francke U. Random X inactivation resulting in mosaic nullisomy of region Xp21.1----p21.3 associated with heterozygosity for ornithine transcarbamylase deficiency and for chronic granulomatous disease. Cytogenet Cell Genet. 1984;38(4):298–307. doi: 10.1159/000132078. [DOI] [PubMed] [Google Scholar]
  4. Goodfellow P., Banting G., Levy R., Povey S., McMichael A. A human X-linked antigen defined by a monoclonal antibody. Somatic Cell Genet. 1980 Nov;6(6):777–787. doi: 10.1007/BF01538976. [DOI] [PubMed] [Google Scholar]
  5. Goodfellow P., Pym B., Mohandas T., Shapiro L. J. The cell surface antigen locus, MIC2X, escapes X-inactivation. Am J Hum Genet. 1984 Jul;36(4):777–782. [PMC free article] [PubMed] [Google Scholar]
  6. Greenstein R. M., Reardon M. P., Chan T. S., Middleton A. B., Mulivor R. A., Greene A. E., Coriell L. L. An (X;11) translocation in a girl with Duchenne muscular dystrophy. Repository identification No. GM1695. Cytogenet Cell Genet. 1980;27(4):268–268. doi: 10.1159/000131496. [DOI] [PubMed] [Google Scholar]
  7. Hu S. Z., Wang T. S., Korn D. DNA primase from KB cells. Evidence for a novel model of primase catalysis by a highly purified primase/polymerase-alpha complex. J Biol Chem. 1984 Feb 25;259(4):2602–2609. [PubMed] [Google Scholar]
  8. Jagadeeswaran P., Lavelle D. E., Kaul R., Mohandas T., Warren S. T. Isolation and characterization of human factor IX cDNA: identification of Taq I polymorphism and regional assignment. Somat Cell Mol Genet. 1984 Sep;10(5):465–473. doi: 10.1007/BF01534851. [DOI] [PubMed] [Google Scholar]
  9. Kafatos F. C., Jones C. W., Efstratiadis A. Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure. Nucleic Acids Res. 1979 Nov 24;7(6):1541–1552. doi: 10.1093/nar/7.6.1541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lindgren V., de Martinville B., Horwich A. L., Rosenberg L. E., Francke U. Human ornithine transcarbamylase locus mapped to band Xp21.1 near the Duchenne muscular dystrophy locus. Science. 1984 Nov 9;226(4675):698–700. doi: 10.1126/science.6494904. [DOI] [PubMed] [Google Scholar]
  11. Meera Khan P. Enzyme electrophoresis on cellulose acetate gel: zymogram patterns in mgh-mouse and man--Chinese hamster somatic cell hybrids. Arch Biochem Biophys. 1971 Aug;145(2):470–483. doi: 10.1016/s0003-9861(71)80007-3. [DOI] [PubMed] [Google Scholar]
  12. Miller O. J., Drayna D., Goodfellow P. Report of the Committee on the Genetic Constitution of the X and Y Chromosomes. Cytogenet Cell Genet. 1984;37(1-4):176–204. doi: 10.1159/000132009. [DOI] [PubMed] [Google Scholar]
  13. Mohandas T., Shapiro L. J., Sparkes R. S., Sparkes M. C. Regional assignment of the steroid sulfatase-X-linked ichthyosis locus: implications for a noninactivated region on the short arm of human X chromosome. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5779–5783. doi: 10.1073/pnas.76.11.5779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mohandas T., Sparkes R. S., Hellkuhl B., Grzeschik K. H., Shapiro L. J. Expression of an X-linked gene from an inactive human X chromosome in mouse-human hybrid cells: further evidence for the noninactivation of the steroid sulfatase locus in man. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6759–6763. doi: 10.1073/pnas.77.11.6759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mohandas T., Sparkes R. S., Shapiro L. J. Genetic evidence for the inactivation of a human autosomal locus attached to an inactive X chromosome. Am J Hum Genet. 1982 Sep;34(5):811–817. [PMC free article] [PubMed] [Google Scholar]
  16. Mohandas T., Sparkes R. S., Shapiro L. J. Reactivation of an inactive human X chromosome: evidence for X inactivation by DNA methylation. Science. 1981 Jan 23;211(4480):393–396. doi: 10.1126/science.6164095. [DOI] [PubMed] [Google Scholar]
  17. Pai G. S., Sprenkle J. A., Do T. T., Mareni C. E., Migeon B. R. Localization of loci for hypoxanthine phosphoribosyltransferase and glucose-6-phosphate dehydrogenase and biochemical evidence of nonrandom X chromosome expression from studies of a human X-autosome translocation. Proc Natl Acad Sci U S A. 1980 May;77(5):2810–2813. doi: 10.1073/pnas.77.5.2810. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schröder J., Nikinmaa B., Kavathas P., Herzenberg L. A. Fluorescence-activated cell sorting of mouse-human hybrid cells aids in locating the gene for the Leu 7 (HNK-1) antigen to human chromosome 11. Proc Natl Acad Sci U S A. 1983 Jun;80(11):3421–3424. doi: 10.1073/pnas.80.11.3421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Seabright M. A rapid banding technique for human chromosomes. Lancet. 1971 Oct 30;2(7731):971–972. doi: 10.1016/s0140-6736(71)90287-x. [DOI] [PubMed] [Google Scholar]
  20. Suomalainen H. A., Goldsby R. A., Osborne B. A., Schröder J. Mouse/human T-cell hybrids rosetting with sheep erythrocytes. Scand J Immunol. 1980;11(2):163–168. doi: 10.1111/j.1365-3083.1980.tb00222.x. [DOI] [PubMed] [Google Scholar]
  21. Tanaka S., Hu S. Z., Wang T. S., Korn D. Preparation and preliminary characterization of monoclonal antibodies against human DNA polymerase alpha. J Biol Chem. 1982 Jul 25;257(14):8386–8390. [PubMed] [Google Scholar]
  22. Wang T. S., Hu S. Z., Korn D. DNA primase from KB cells. Characterization of a primase activity tightly associated with immunoaffinity-purified DNA polymerase-alpha. J Biol Chem. 1984 Feb 10;259(3):1854–1865. [PubMed] [Google Scholar]
  23. Wyandt H. E., Wysham D. G., Minden S. K., Anderson R. S., Hecht F. Mechanisms of giemsa banding of chromosomes. I. Giemsa-11 banding with azure and eosin. Exp Cell Res. 1976 Oct 1;102(1):85–94. doi: 10.1016/0014-4827(76)90302-5. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES