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. 1985 Aug;82(16):5328–5331. doi: 10.1073/pnas.82.16.5328

Actinomycin and DNA transcription.

H M Sobell
PMCID: PMC390561  PMID: 2410919

Abstract

Recent advances in understanding how actinomycin binds to DNA have suggested its mechanism of action. Actinomycin binds to a premelted DNA conformation present within the transcriptional complex. This immobilizes the complex, interfering with the elongation of growing RNA chains. The model has a number of implications for understanding RNA synthesis.

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Selected References

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  1. Banerjee A., Sobell H. M. Presence of nonlinear excitations in DNA structure and their relationship to DNA premelting and to drug intercalation. J Biomol Struct Dyn. 1983 Oct;1(1):253–262. doi: 10.1080/07391102.1983.10507438. [DOI] [PubMed] [Google Scholar]
  2. Brahms J. G., Dargouge O., Brahms S., Ohara Y., Vagner V. Activation and inhibition of transcription by supercoiling. J Mol Biol. 1985 Feb 20;181(4):455–465. doi: 10.1016/0022-2836(85)90419-x. [DOI] [PubMed] [Google Scholar]
  3. Cartwright I. L., Elgin S. C. Analysis of chromatin structure and DNA sequence organization: use of the 1,10-phenanthroline-cuprous complex. Nucleic Acids Res. 1982 Oct 11;10(19):5835–5852. doi: 10.1093/nar/10.19.5835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chamberlin M. J. The selectivity of transcription. Annu Rev Biochem. 1974;43(0):721–775. doi: 10.1146/annurev.bi.43.070174.003445. [DOI] [PubMed] [Google Scholar]
  5. Crothers D. M. Calculation of binding isotherms for heterogenous polymers. Biopolymers. 1968 Apr;6(4):575–584. doi: 10.1002/bip.1968.360060411. [DOI] [PubMed] [Google Scholar]
  6. Darlix J. L., Fromageot P. Discontinuous in vitro transcription of DNA. Biochimie. 1972;54(1):47–54. doi: 10.1016/s0300-9084(72)80037-3. [DOI] [PubMed] [Google Scholar]
  7. GOLDBERG I. H., RABINOWITZ M. Actionmycin D inhibition of deoxyribonucleic acid-dependent synthesis of ribonucleic acid. Science. 1962 Apr 27;136(3513):315–316. doi: 10.1126/science.136.3513.315. [DOI] [PubMed] [Google Scholar]
  8. GOLDBERG I. H., RABINOWITZ M., REICH E. Basis of actinomycin action. I. DNA binding and inhibition of RNA-polymerase synthetic reactions by actinomycin. Proc Natl Acad Sci U S A. 1962 Dec 15;48:2094–2101. doi: 10.1073/pnas.48.12.2094. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Glikin G. C., Gargiulo G., Rena-Descalzi L., Worcel A. Escherichia coli single-strand binding protein stabilizes specific denatured sites in superhelical DNA. Nature. 1983 Jun 30;303(5920):770–774. doi: 10.1038/303770a0. [DOI] [PubMed] [Google Scholar]
  10. Jain S. C., Sobell H. M. Stereochemistry of actinomycin binding to DNA. I. Refinement and further structural details of the actinomycin-deoxyguanosine crystalline complex. J Mol Biol. 1972 Jul 14;68(1):1–20. doi: 10.1016/0022-2836(72)90258-6. [DOI] [PubMed] [Google Scholar]
  11. Jessee B., Gargiulo G., Razvi F., Worcel A. Analogous cleavage of DNA by micrococcal nuclease and a 1-10-phenanthroline-cuprous complex. Nucleic Acids Res. 1982 Oct 11;10(19):5823–5834. doi: 10.1093/nar/10.19.5823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. KIRK J. M. The mode of action of actinomycin D. Biochim Biophys Acta. 1960 Jul 29;42:167–169. doi: 10.1016/0006-3002(60)90769-1. [DOI] [PubMed] [Google Scholar]
  13. Maderious A., Chen-Kiang S. Pausing and premature termination of human RNA polymerase II during transcription of adenovirus in vivo and in vitro. Proc Natl Acad Sci U S A. 1984 Oct;81(19):5931–5935. doi: 10.1073/pnas.81.19.5931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Maizels N. M. The nucleotide sequence of the lactose messenger ribonucleic acid transcribed from the UV5 promoter mutant of Escherichia coli. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3585–3589. doi: 10.1073/pnas.70.12.3585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McClure W. R., Hawley D. K., Youderian P., Susskind M. M. DNA determinants of promoter selectivity in Escherichia coli. Cold Spring Harb Symp Quant Biol. 1983;47(Pt 1):477–481. doi: 10.1101/sqb.1983.047.01.057. [DOI] [PubMed] [Google Scholar]
  16. Miller O. L., Jr, Hamkalo B. A. Visualization of RNA synthesis on chromosomes. Int Rev Cytol. 1972;33:1–25. doi: 10.1016/s0074-7696(08)61446-1. [DOI] [PubMed] [Google Scholar]
  17. Reich E., Goldberg I. H. Actinomycin and nucleic acid function. Prog Nucleic Acid Res Mol Biol. 1964;3:183–234. doi: 10.1016/s0079-6603(08)60742-4. [DOI] [PubMed] [Google Scholar]
  18. Roberts J. W. Transcription termination and late control in phage lambda. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3300–3304. doi: 10.1073/pnas.72.9.3300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ryoji M., Worcel A. Chromatin assembly in Xenopus oocytes: in vivo studies. Cell. 1984 May;37(1):21–32. doi: 10.1016/0092-8674(84)90297-6. [DOI] [PubMed] [Google Scholar]
  20. Sentenac A., Simon E. J., Fromageot P. Initiation of chains by RNA polymerase and the effects of inhibitors studied by a direct filtration technique. Biochim Biophys Acta. 1968 Jul 23;161(2):299–308. doi: 10.1016/0005-2787(68)90108-1. [DOI] [PubMed] [Google Scholar]
  21. Sobell H. M. How actinomycin binds to DNA. Sci Am. 1974 Aug;231(2):82–91. doi: 10.1038/scientificamerican0874-82. [DOI] [PubMed] [Google Scholar]
  22. Sobell H. M., Jain S. C., Sakore T. D., Nordman C. E. Stereochemistry of actinomycin--DNA binding. Nat New Biol. 1971 Jun 16;231(24):200–205. doi: 10.1038/newbio231200a0. [DOI] [PubMed] [Google Scholar]
  23. Sobell H. M., Jain S. C. Stereochemistry of actinomycin binding to DNA. II. Detailed molecular model of actinomycin-DNA complex and its implications. J Mol Biol. 1972 Jul 14;68(1):21–34. doi: 10.1016/0022-2836(72)90259-8. [DOI] [PubMed] [Google Scholar]
  24. Sobell H. M., Tsai C. C., Jain S. C., Gilbert S. G. Visualization of drug-nucleic acid interactions at atomic resolution. III. Unifying structural concepts in understanding drug-DNA interactions and their broader implications in understanding protein-DNA interactions. J Mol Biol. 1977 Aug 15;114(3):333–365. doi: 10.1016/0022-2836(77)90254-6. [DOI] [PubMed] [Google Scholar]
  25. Yanofsky C. Attenuation in the control of expression of bacterial operons. Nature. 1981 Feb 26;289(5800):751–758. doi: 10.1038/289751a0. [DOI] [PubMed] [Google Scholar]

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