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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1985 Aug;82(16):5418–5422. doi: 10.1073/pnas.82.16.5418

P-element distribution in Eurasian populations of Drosophila melanogaster: A genetic and molecular analysis

Dominique Anxolabéhère , Danielle Nouaud , Georges Périquet , Paul Tchen §
PMCID: PMC390580  PMID: 16593591

Abstract

Genetic and molecular investigations were carried out with Eurasian Drosophila melanogaster populations on the P-M system of hybrid dysgenesis. In 27 strains sampled from France to Middle Asia, a clear gradient exists between Western Europe, in which most modern strains are of the Q type, and eastern areas, in which M-cytotype strains predominate. Molecular analysis on individual flies was performed with two complementary probes of the cloned 2.9-kilobase P element. The results provide evidence for a gradually decreasing frequency of P elements from west to east, but the presence of P-homologous sequences has been ascertained in all of the wild M-cytotype populations analyzed. Moreover, some active P elements with GD sterility potential were revealed in the majority of M-cytotype populations when tested with a highly sensitive reference line. The gradual change in distribution of the polymorphic P family in Eurasia is discussed in relation to the structure of the elements together with the theories of P-M evolution and is interpreted as the present invasion of Eurasian populations by these elements.

Keywords: transposable elements, hybrid dysgenesis, DNA hybridization, geographical variation

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Selected References

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  1. Anxolabéhère D., Nouaud D., Périquet G. Cytotype polymorphism of the P-M system in two wild populations of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1982 Dec;79(24):7801–7803. doi: 10.1073/pnas.79.24.7801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bingham P. M., Kidwell M. G., Rubin G. M. The molecular basis of P-M hybrid dysgenesis: the role of the P element, a P-strain-specific transposon family. Cell. 1982 Jul;29(3):995–1004. doi: 10.1016/0092-8674(82)90463-9. [DOI] [PubMed] [Google Scholar]
  3. Brookfield J. F., Montgomery E., Langley C. H. Apparent absence of transposable elements related to the P elements of D. melanogaster in other species of Drosophila. 1984 Jul 26-Aug 1Nature. 310(5975):330–332. doi: 10.1038/310330a0. [DOI] [PubMed] [Google Scholar]
  4. Bucheton A., Paro R., Sang H. M., Pelisson A., Finnegan D. J. The molecular basis of I-R hybrid dysgenesis in Drosophila melanogaster: identification, cloning, and properties of the I factor. Cell. 1984 Aug;38(1):153–163. doi: 10.1016/0092-8674(84)90536-1. [DOI] [PubMed] [Google Scholar]
  5. Daniels S. B., Strausbaugh L. D., Ehrman L., Armstrong R. Sequences homologous to P elements occur in Drosophila paulistorum. Proc Natl Acad Sci U S A. 1984 Nov;81(21):6794–6797. doi: 10.1073/pnas.81.21.6794. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Engels W. R. A trans-acting product needed for P factor transposition in Drosophila. Science. 1984 Dec 7;226(4679):1194–1196. doi: 10.1126/science.6095450. [DOI] [PubMed] [Google Scholar]
  7. Engels W. R. Germline hypermutability in Drosophila and its relation to hybrid dysgenesis and cytotype. Genetics. 1981 Jul;98(3):565–587. doi: 10.1093/genetics/98.3.565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Engels W. R. The P family of transposable elements in Drosophila. Annu Rev Genet. 1983;17:315–344. doi: 10.1146/annurev.ge.17.120183.001531. [DOI] [PubMed] [Google Scholar]
  9. Kidwell M. G. Evolution of hybrid dysgenesis determinants in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1983 Mar;80(6):1655–1659. doi: 10.1073/pnas.80.6.1655. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kidwell M. G., Kidwell J. F., Sved J. A. Hybrid Dysgenesis in DROSOPHILA MELANOGASTER: A Syndrome of Aberrant Traits Including Mutation, Sterility and Male Recombination. Genetics. 1977 Aug;86(4):813–833. doi: 10.1093/genetics/86.4.813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. O'Hare K., Rubin G. M. Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genome. Cell. 1983 Aug;34(1):25–35. doi: 10.1016/0092-8674(83)90133-2. [DOI] [PubMed] [Google Scholar]
  12. Rubin G. M., Kidwell M. G., Bingham P. M. The molecular basis of P-M hybrid dysgenesis: the nature of induced mutations. Cell. 1982 Jul;29(3):987–994. doi: 10.1016/0092-8674(82)90462-7. [DOI] [PubMed] [Google Scholar]
  13. Schaefer R. E., Kidwell M. G., Fausto-Sterling A. Hybrid Dysgenesis in DROSOPHILA MELANOGASTER: Morphological and Cytological Studies of Ovarian Dysgenesis. Genetics. 1979 Aug;92(4):1141–1152. doi: 10.1093/genetics/92.4.1141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Simmons M. J., Raymond J. D., Culbert T. P., Laverty T. R. Analysis of dysgenesis-induced lethal mutations on the X chromosome of a Q strain of Drosophila melanogaster. Genetics. 1984 May;107(1):49–63. doi: 10.1093/genetics/107.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tchen P., Fuchs R. P., Sage E., Leng M. Chemically modified nucleic acids as immunodetectable probes in hybridization experiments. Proc Natl Acad Sci U S A. 1984 Jun;81(11):3466–3470. doi: 10.1073/pnas.81.11.3466. [DOI] [PMC free article] [PubMed] [Google Scholar]

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