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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1985 Sep;82(17):5641–5645. doi: 10.1073/pnas.82.17.5641

Molecular cloning of an activated human oncogene, homologous to v-raf, from primary stomach cancer.

K Shimizu, Y Nakatsu, M Sekiguchi, K Hokamura, K Tanaka, M Terada, T Sugimura
PMCID: PMC390607  PMID: 3862088

Abstract

Transfection with high molecular weight DNA from a primary stomach cancer induced foci of transformed NIH 3T3 cells, and the transformed cells were tumorigenic in nude mice. By screening with a human Alu-family probe, we isolated the human DNA sequence from the secondary transformant cells. This transforming sequence encompasses about 60 kilobase pairs and is unrelated to known human transforming genes. Examination of homologies between this sequence and retroviral oncogenes revealed that the human transforming sequence is closely related to the v-raf oncogene of murine transforming retrovirus 3611-MSV.

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Selected References

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