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. 1985 Sep;82(17):5751–5755. doi: 10.1073/pnas.82.17.5751

beta-Endorphin: surface binding and internalization in thymoma cells.

L Schweigerer, W Schmidt, H Teschemacher, C Gramsch
PMCID: PMC390630  PMID: 2994047

Abstract

The opioid peptide beta-endorphin binds to specific nonopioid binding sites (Mr 72,000) that are present on the surface of thymoma cells. beta-Endorphin is then internalized, apparently via the Mr 72,000 species, and is subsequently found within intracellular, vesicular structures. This process is accompanied by the down-regulation of the Mr 72,000 binding sites. Our findings suggest that beta-endorphin may modulate cellular functions, such as T-lymphocyte proliferation, at intracellular rather than cell surface sites.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blanchard S. G., Chang K. J., Cuatrecasas P. Characterization of the association of tritiated enkephalin with neuroblastoma cells under conditions optimal for receptor down regulation. J Biol Chem. 1983 Jan 25;258(2):1092–1097. [PubMed] [Google Scholar]
  2. Branca A. A., Faltynek C. R., D'Alessandro S. B., Baglioni C. Interaction of interferon with cellular receptors. Internalization and degradation of cell-bound interferon. J Biol Chem. 1982 Nov 25;257(22):13291–13296. [PubMed] [Google Scholar]
  3. Caro J. F., Muller G., Glennon J. A. Insulin processing by the liver. J Biol Chem. 1982 Jul 25;257(14):8459–8466. [PubMed] [Google Scholar]
  4. Carpenter G., Cohen S. 125I-labeled human epidermal growth factor. Binding, internalization, and degradation in human fibroblasts. J Cell Biol. 1976 Oct;71(1):159–171. doi: 10.1083/jcb.71.1.159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chang K. J., Eckel R. W., Blanchard S. G. Opioid peptides induce reduction of enkephalin receptors in cultured neuroblastoma cells. Nature. 1982 Apr 1;296(5856):446–448. doi: 10.1038/296446a0. [DOI] [PubMed] [Google Scholar]
  6. Festenstein H., Schmidt W. Variation in MHC antigenic profiles of tumor cells and its biological effects. Immunol Rev. 1981;60:85–127. doi: 10.1111/j.1600-065x.1981.tb00363.x. [DOI] [PubMed] [Google Scholar]
  7. Gilman S. C., Schwartz J. M., Milner R. J., Bloom F. E., Feldman J. D. beta-Endorphin enhances lymphocyte proliferative responses. Proc Natl Acad Sci U S A. 1982 Jul;79(13):4226–4230. doi: 10.1073/pnas.79.13.4226. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Goldstein J. L., Anderson R. G., Brown M. S. Coated pits, coated vesicles, and receptor-mediated endocytosis. Nature. 1979 Jun 21;279(5715):679–685. doi: 10.1038/279679a0. [DOI] [PubMed] [Google Scholar]
  9. Gramsch C., Meo T., Riethmüller G., Herz A. Binding characteristics of a monoclonal beta-endorphin antibody recognizing the N-terminus of opioid peptides. J Neurochem. 1983 May;40(5):1220–1226. doi: 10.1111/j.1471-4159.1983.tb13560.x. [DOI] [PubMed] [Google Scholar]
  10. Guillemin R., Vargo T., Rossier J., Minick S., Ling N., Rivier C., Vale W., Bloom F. beta-Endorphin and adrenocorticotropin are selected concomitantly by the pituitary gland. Science. 1977 Sep 30;197(4311):1367–1369. doi: 10.1126/science.197601. [DOI] [PubMed] [Google Scholar]
  11. Haigler H. T., Maxfield F. R., Willingham M. C., Pastan I. Dansylcadaverine inhibits internalization of 125I-epidermal growth factor in BALB 3T3 cells. J Biol Chem. 1980 Feb 25;255(4):1239–1241. [PubMed] [Google Scholar]
  12. Hazum E., Chang K. J., Cuatrecasas P. Interaction of iodinated human [D-Ala2]beta-endorphin with opitate receptors. J Biol Chem. 1979 Mar 25;254(6):1765–1767. [PubMed] [Google Scholar]
  13. Hazum E., Chang K. J., Cuatrecasas P. Specific nonopiate receptors for beta-endorphin. Science. 1979 Sep 7;205(4410):1033–1035. doi: 10.1126/science.224457. [DOI] [PubMed] [Google Scholar]
  14. Kelly K., Cochran B. H., Stiles C. D., Leder P. Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor. Cell. 1983 Dec;35(3 Pt 2):603–610. doi: 10.1016/0092-8674(83)90092-2. [DOI] [PubMed] [Google Scholar]
  15. King A. C., Cuatrecasas P. Peptide hormone-induced receptor mobility, aggregation, and internalization. N Engl J Med. 1981 Jul 9;305(2):77–88. doi: 10.1056/NEJM198107093050206. [DOI] [PubMed] [Google Scholar]
  16. Kruijer W., Cooper J. A., Hunter T., Verma I. M. Platelet-derived growth factor induces rapid but transient expression of the c-fos gene and protein. Nature. 1984 Dec 20;312(5996):711–716. doi: 10.1038/312711a0. [DOI] [PubMed] [Google Scholar]
  17. Law P. Y., Hom D. S., Loh H. H. Down-regulation of opiate receptor in neuroblastoma x glioma NG108-15 hybrid cells. Chloroquine promotes accumulation of tritiated enkephalin in the lysosomes. J Biol Chem. 1984 Apr 10;259(7):4096–4104. [PubMed] [Google Scholar]
  18. Li C. H. beta-Endorphin. Cell. 1982 Dec;31(3 Pt 2):504–505. doi: 10.1016/0092-8674(82)90305-1. [DOI] [PubMed] [Google Scholar]
  19. McCain H. W., Lamster I. B., Bozzone J. M., Grbic J. T. Beta-endorphin modulates human immune activity via non-opiate receptor mechanisms. Life Sci. 1982 Oct 11;31(15):1619–1624. doi: 10.1016/0024-3205(82)90054-6. [DOI] [PubMed] [Google Scholar]
  20. Müller R., Bravo R., Burckhardt J., Curran T. Induction of c-fos gene and protein by growth factors precedes activation of c-myc. Nature. 1984 Dec 20;312(5996):716–720. doi: 10.1038/312716a0. [DOI] [PubMed] [Google Scholar]
  21. Pastan I. H., Willingham M. C. Receptor-mediated endocytosis of hormones in cultured cells. Annu Rev Physiol. 1981;43:239–250. doi: 10.1146/annurev.ph.43.030181.001323. [DOI] [PubMed] [Google Scholar]
  22. Pilch P. F., Czech M. P. The subunit structure of the high affinity insulin receptor. Evidence for a disulfide-linked receptor complex in fat cell and liver plasma membranes. J Biol Chem. 1980 Feb 25;255(4):1722–1731. [PubMed] [Google Scholar]
  23. Sando J. J., Young M. C. Identification of high-affinity phorbol ester receptor in cytosol of EL4 thymoma cells: requirement for calcium, magnesium, and phospholipids. Proc Natl Acad Sci U S A. 1983 May;80(9):2642–2646. doi: 10.1073/pnas.80.9.2642. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Schweigerer L., Bhakdi S., Teschemacher H. Specific non-opiate binding sites for human beta-endorphin on the terminal complex of human complement. Nature. 1982 Apr 8;296(5857):572–574. doi: 10.1038/296572a0. [DOI] [PubMed] [Google Scholar]
  25. Schweigerer L., Teschemacher H., Bhakdi S., Lederle M. Interaction of human beta-endorphin with nonopiate binding sites on the terminal SC5b-9 complex of human complement. Significance of COOH-terminal beta H-endorphin fragments. J Biol Chem. 1983 Oct 25;258(20):12287–12292. [PubMed] [Google Scholar]
  26. Silverstein S. C., Steinman R. M., Cohn Z. A. Endocytosis. Annu Rev Biochem. 1977;46:669–722. doi: 10.1146/annurev.bi.46.070177.003321. [DOI] [PubMed] [Google Scholar]
  27. Westphal M., Li C. H. beta-Endorphin: evidence for the existence of opioid and non-opioid binding components for the tritiated human hormone in NG108-15 cells. Biochem Biophys Res Commun. 1984 Jul 18;122(1):428–433. doi: 10.1016/0006-291x(84)90493-5. [DOI] [PubMed] [Google Scholar]

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