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. 1985 Sep;82(17):5832–5835. doi: 10.1073/pnas.82.17.5832

Chromosomal localization of the human Thy-1 gene.

J van Rijs, V Giguère, J Hurst, T van Agthoven, A Geurts van Kessel, S Goyert, F Grosveld
PMCID: PMC390647  PMID: 2863819

Abstract

We have isolated the gene coding for human Thy-1. Introduction of this gene into HeLa cells by DNA-mediated transfer results in the expression of Thy-1 antigen on the cell surface. Chromosomal mapping of the Thy-1 gene by hybridization to metaphase chromosomes and Southern blots of DNA from hybrid cells indicate that the Thy-1 gene is located on the long arm of chromosome 11.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bartram C. R., de Klein A., Hagemeijer A., van Agthoven T., Geurts van Kessel A., Bootsma D., Grosveld G., Ferguson-Smith M. A., Davies T., Stone M. Translocation of c-ab1 oncogene correlates with the presence of a Philadelphia chromosome in chronic myelocytic leukaemia. Nature. 1983 Nov 17;306(5940):277–280. doi: 10.1038/306277a0. [DOI] [PubMed] [Google Scholar]
  2. Campbell D. G., Gagnon J., Reid K. B., Williams A. F. Rat brain Thy-1 glycoprotein. The amino acid sequence, disulphide bonds and an unusual hydrophobic region. Biochem J. 1981 Apr 1;195(1):15–30. doi: 10.1042/bj1950015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cohen F. E., Novotný J., Sternberg M. J., Campbell D. G., Williams A. F. Analysis of structural similarities between brain Thy-1 antigen and immunoglobulin domains. Evidence for an evolutionary relationship and a hypothesis for its functional significance. Biochem J. 1981 Apr 1;195(1):31–40. doi: 10.1042/bj1950031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Evans G. A., Ingraham H. A., Lewis K., Cunningham K., Seki T., Moriuchi T., Chang H. C., Silver J., Hyman R. Expression of the Thy-1 glycoprotein gene by DNA-mediated gene transfer. Proc Natl Acad Sci U S A. 1984 Sep;81(17):5532–5536. doi: 10.1073/pnas.81.17.5532. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Graham F. L., van der Eb A. J. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology. 1973 Apr;52(2):456–467. doi: 10.1016/0042-6822(73)90341-3. [DOI] [PubMed] [Google Scholar]
  6. Grosveld F. G., Lund T., Murray E. J., Mellor A. L., Dahl H. H., Flavell R. A. The construction of cosmid libraries which can be used to transform eukaryotic cells. Nucleic Acids Res. 1982 Nov 11;10(21):6715–6732. doi: 10.1093/nar/10.21.6715. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Grosveld G. C., de Boer E., Shewmaker C. K., Flavell R. A. DNA sequences necessary for transcription of the rabbit beta-globin gene in vivo. Nature. 1982 Jan 14;295(5845):120–126. doi: 10.1038/295120a0. [DOI] [PubMed] [Google Scholar]
  8. Hagemeijer A., Smit E. M., Bootsma D. Improved identification of chromosomes of leukemic cells in methotrexate-treated cultures. Cytogenet Cell Genet. 1979;23(3):208–212. doi: 10.1159/000131327. [DOI] [PubMed] [Google Scholar]
  9. Hood L., Kronenberg M., Hunkapiller T. T cell antigen receptors and the immunoglobulin supergene family. Cell. 1985 Feb;40(2):225–229. doi: 10.1016/0092-8674(85)90133-3. [DOI] [PubMed] [Google Scholar]
  10. Ish-Horowicz D., Burke J. F. Rapid and efficient cosmid cloning. Nucleic Acids Res. 1981 Jul 10;9(13):2989–2998. doi: 10.1093/nar/9.13.2989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jeffreys A. J., Flavell R. A. A physical map of the DNA regions flanking the rabbit beta-globin gene. Cell. 1977 Oct;12(2):429–439. doi: 10.1016/0092-8674(77)90119-2. [DOI] [PubMed] [Google Scholar]
  12. Kemshead J. T., Ritter M. A., Cotmore S. F., Greaves M. F. Human Thy-1: expression on the cell surface of neuronal and glial cells. Brain Res. 1982 Mar 25;236(2):451–461. doi: 10.1016/0006-8993(82)90727-2. [DOI] [PubMed] [Google Scholar]
  13. Maino V. C., Norcross M. A., Perkins M. S., Smith R. T. Mechanism of Thy-1-mediated T cell activation: roles of Fc receptors, T200, Ia, and H-2 glycoproteins in accessory cell function. J Immunol. 1981 May;126(5):1829–1836. [PubMed] [Google Scholar]
  14. Martiniuk F., Smith M., Ellenbogen A., Desnick R. J., Astrin K., Mitra J., Hirschhorn R. Assignment of the gene for neutral alpha-glucosidase AB to chromosome 11. Cytogenet Cell Genet. 1983;35(2):110–116. doi: 10.1159/000131851. [DOI] [PubMed] [Google Scholar]
  15. McKenzie J. L., Fabre J. W. Human thy-1: unusual localization and possible functional significance in lymphoid tissues. J Immunol. 1981 Mar;126(3):843–850. [PubMed] [Google Scholar]
  16. McKenzie J. L., Fabre J. W. Human thy-1: unusual localization and possible functional significance in lymphoid tissues. J Immunol. 1981 Mar;126(3):843–850. [PubMed] [Google Scholar]
  17. Moriuchi T., Chang H. C., Denome R., Silver J. Thy-1 cDNA sequence suggests a novel regulatory mechanism. Nature. 1983 Jan 6;301(5895):80–82. doi: 10.1038/301080a0. [DOI] [PubMed] [Google Scholar]
  18. Parker B. A., Stark G. R. Regulation of simian virus 40 transcription: sensitive analysis of the RNA species present early in infections by virus or viral DNA. J Virol. 1979 Aug;31(2):360–369. doi: 10.1128/jvi.31.2.360-369.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. REIF A. E., ALLEN J. M. THE AKR THYMIC ANTIGEN AND ITS DISTRIBUTION IN LEUKEMIAS AND NERVOUS TISSUES. J Exp Med. 1964 Sep 1;120:413–433. doi: 10.1084/jem.120.3.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schlesinger H. R., Gerson J. M., Moorhead P. S., Maguire H., Hummeler K. Establishment and characterization of human neuroblastoma cell lines. Cancer Res. 1976 Sep;36(9 PT1):3094–3100. [PubMed] [Google Scholar]
  21. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  22. Williams A. F., Gagnon J. Neuronal cell Thy-1 glycoprotein: homology with immunoglobulin. Science. 1982 May 14;216(4547):696–703. doi: 10.1126/science.6177036. [DOI] [PubMed] [Google Scholar]
  23. Williams A. F. Surface molecules and cell interactions. J Theor Biol. 1982 Sep 21;98(2):221–234. doi: 10.1016/0022-5193(82)90260-0. [DOI] [PubMed] [Google Scholar]
  24. Williams C. A., Barna J., Schupf N. Antibody to Thy-1 antigen injected into rat hypothalamus selectively inhibits carbamyl choline induced drinking. Nature. 1980 Jan 3;283(5742):82–84. doi: 10.1038/283082a0. [DOI] [PubMed] [Google Scholar]
  25. van Kessel A. G., Tetteroo P., van Agthoven T., Paulussen R., van Dongen J., Hagemeijer A., von dem Borne A. Localization of human myeloid-associated surface antigen detected by a panel of 20 monoclonal antibodies to the q12-qter region of chromosome 11. J Immunol. 1984 Sep;133(3):1265–1269. [PubMed] [Google Scholar]

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