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. 1985 Sep;82(17):5875–5879. doi: 10.1073/pnas.82.17.5875

Two-micrometer circle site-specific recombination: the minimal substrate and the possible role of flanking sequences.

M Jayaram
PMCID: PMC390656  PMID: 3898075

Abstract

The 2-mum circle DNA of yeast encodes a site-specific recombination system (FLP recombination). The recombination region had been mapped earlier to a 65-base-pair (bp) segment within the 599-bp-long inverted repeats of the molecule. I have shown that the "minimal" FLP substrate resides in a 13-bp dyad symmetry plus an 8-bp core located within the 65-bp recombination region. Further, as determined by different in vivo assays, sequences extraneous to the minimal FLP site and the 65-bp recombination region can affect the efficiency of the recombination reaction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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