Abstract
Three distinct forms of human alkaline phosphatase, presumably isozymes, are known, each apparently associated with a specific tissue. These are placental, intestinal, and liver (kidney and bone). We have used a specific immunoassay and HPLC to show that placental alkaline phosphatase is also present in extracts of liver and intestine in appreciable amounts.
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- Ezra E., Blacher R., Udenfriend S. Purification and partial sequencing of human placental alkaline phosphatase. Biochem Biophys Res Commun. 1983 Nov 15;116(3):1076–1083. doi: 10.1016/s0006-291x(83)80252-6. [DOI] [PubMed] [Google Scholar]
- Gogolin K. J., Wray L. K., Slaughter C. A., Harris H. A monoclonal antibody that reacts with nonallelic enzyme glycoproteins. Science. 1982 Apr 2;216(4541):59–61. doi: 10.1126/science.6175022. [DOI] [PubMed] [Google Scholar]
- Kitagawa T., Aikawa T. Enzyme coupled immunoassay of insulin using a novel coupling reagent. J Biochem. 1976 Jan;79(1):233–236. doi: 10.1093/oxfordjournals.jbchem.a131053. [DOI] [PubMed] [Google Scholar]
- McKenna M. J., Hamilton T. A., Sussman H. H. Comparison of human alkaline phosphatase isoenzymes. Structural evidence for three protein classes. Biochem J. 1979 Jul 1;181(1):67–73. doi: 10.1042/bj1810067. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mulivor R. A., Hannig V. L., Harris H. Developmental change in human intestinal alkaline phosphatase. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3909–3912. doi: 10.1073/pnas.75.8.3909. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mulivor R. A., Plotkin L. I., Harris H. Differential inhibition of the products of the human alkaline phosphatase loci. Ann Hum Genet. 1978 Jul;42(1):1–13. doi: 10.1111/j.1469-1809.1978.tb00927.x. [DOI] [PubMed] [Google Scholar]
- Seargeant L. E., Stinson R. A. Evidence that three structural genes code for human alkaline phosphatases. Nature. 1979 Sep 13;281(5727):152–154. doi: 10.1038/281152a0. [DOI] [PubMed] [Google Scholar]
- Slaughter C. A., Gogolin K. J., Coseo M. C., Meyer L. J., Lesko J., Harris H. Discrimination of human placental alkaline phosphatase allelic variants by monoclonal antibodies. Am J Hum Genet. 1983 Jan;35(1):1–20. [PMC free article] [PubMed] [Google Scholar]
- Trépanier J. M., Seargeant L. E., Stinson R. A. Affinity purification and some molecular properties of human liver alkaline phosphatase. Biochem J. 1976 Jun 1;155(3):653–660. doi: 10.1042/bj1550653. [DOI] [PMC free article] [PubMed] [Google Scholar]