Table I.
GT-B GTs of known 3D structure
| GT-B Family | Catalytic mechanism | Membrane associated | Nonmembrane associated |
|---|---|---|---|
| GT1 | Inverting |
Alg13/Alg14 (PMa,
apo, Wang et al.
2008), Ugt2b7 (BTb, apo, Miley et al. 2007) |
CalG1/CalG2/CalG4, CalG3, EryCIII, GtfA, GtfB, GtfD, OGT/NGT, OleD/OleI, SpnG, Ufgt, UGT71G1, UGT78G1, UGT85H2, UrdGT2 |
| GT3 | Retaining | – | Gsy2 |
| GT4 | Retaining | AviGT4 (apo and UDP, Martinez-Fleites et al. 2006), CGT (MT, apo, Lee, Lee et al. 2011; Lee, Hong et al. 2011), PimA (PM, GDP and GDP-Man, Guerin et al. 2007), PimB’ (PM, GDP and GDP-Man, Batt et al. 2010), SUS1 (PM, UDP-Glc, UDP/Fru, Zheng et al. 2011), WaaG (MTc, UDP and UDP-2F-Glc, Martinez-Fleites et al. 2006), WsaF (PM, apo, dTDP and dTDP-Rha, Steiner et al. 2010), WbaZ (MT, apo, Liu et al. 1993) | BshA, MshA, NY2A_B736L, SpsA, TreT |
| GT5 | Retaining | – | AtGlgA, EcGlgA, PaGlgA, OsGBSSI, HvSSI |
| GT9 | Inverting |
WaaC (MT, apo, ADP and
ADP-2F-Hep, Grizot et al. 2006),
WaaF (MT, apo, pdb code
1PSW), Vpar_0760 (MT, apo, to be published) |
– |
| GT10 | Inverting | FucT (PM, apo, GDP and GDP-Fuc, Sun et al. 2007) | – |
| GT20 | Retaining | – | OtsA |
| GT23 | Inverting | FUT8 (BTb, apo, Ihara et al. 2007) | NodZ |
| GT28 | Inverting | MurG (MT, apo and UDP-GlcNAc, Hu et al. 2003; Brown et al. 2013) | – |
| GT30 | Inverting | WaaA (MT, apo and CMP, Schmidt et al. 2012) | – |
| GT35 | Retaining | – | GP (liver/muscle), Gph1, MalP, SP |
| GT41 | Inverting | – | HsOGT, XcOGT, HMW1C |
| GT52 | Inverting | NST (BT, apo, CMP, CDP and CMP-3F-Neu5Ac, Lin et al. 2011) | – |
| GT63 | Inverting | – | BGT |
| GT65 | Inverting | – | PoFUT1 |
| GT68 | Inverting | – | PoFUT2 |
| GT70 | Inverting | GumK (MT, apo, UDP, Barreras et al. 2008) | – |
| GT72 | Retaining | – | AGT |
| GT80 | Inverting |
PmST1 (BT, CMP and
CMP-3F-Neu5Ac, CMP-3F-Neu5Ac/Lac, CMP/Lac, Ni et al. 2007; Kim et al. 2008), ST (MT, CMP, Iwatani et al. 2009; Tsukamoto et al. 2007), Pst6-224 (MT, CMP, Kakuta et al. 2008) |
– |
aPM, peripheral GT; bBT, bitopic GT; cMT, monotopic GT; dn.d., not determined. TagF was classified as a GT2 enzyme due to the presence of both GT-A and GT-B folds in the GTs.
Alg13, UDP-GlcNAc:Dol-PP-GlcNAc N-acetylglucosaminyltransferase; Ugt2b7, UDP-GlcA:β-glucuronosyltransferase 2B7; CalG1, calicheamicin GT 1; CalG2, calicheamicin GT 2; CalG3, calicheamicin GT 3; CalG4, calicheamicin GT 4; EryCIII, TDP-desosamine:α-mycarosyl erythronolide B desosaminyltransferase; GtfA, dTDP-β-L-4-epi-epivancosamine:epivancosaminyltransferase; GtfB, TDP/UDP-glucose:aglycosyl-vancomycin glucosyltransferase; GtfD, UDP-β-l-4-epi-vancosamine:vancomycin-pseudoaglycone vancosaminyltransferase; OGT/NGT, UDP-Glc:sinapoyl-alcohol-, 2,5-DHBA-, 3,4-DHBA-glucosyltransferase; OleD, oleandomycin GT; OleI, oleandomycin GT; SpnG, TDP-β-l-Rha:spynosin 9-O-α-l-rhamnosyltransferase; Ufgt, UDP-Glc: Anthocyanidin 3-O-glucosyltransferase; UGT71G1, UDP-Glc:flavonoid β-glucosyltransferase; UGT78G1, UDP-glucose:flavonoid β–glucosyltransferase; UGT85H2, UDP-glucose:(iso)flavonoid β–glucosyltransferase; UrdGT2, urdamycin A GT II; Gsy2, glycogen synthase; CGT, cholesterol α-glucosyltransferase; PimA, GDP-Man:phosphatidylinositol mannosyltransferase; PimB’, GDP-Man: Phosphatidylinositolmannose mannosyltransferase; WaaG, UDP-Glc:l-glycero-d-manno-heptose II -1,3-glucosyltransferase I; WbaZ, putative mannosyltransferase; WsaF, TDP-β-L-Rha; S-Layer glycoprotein β-1,2-rhamnosyltransferase; WaaF, heptosyltransferase II; BshA, UDP-GlcNAc: l-malate α-N-acetylglucosaminyltransferase; MshA, UDP-GlcNAc:inositol-P N-acetylglucosaminyltransferase; TreT, trehalose synthase; SpsA, sucrose phosphate synthase; AviGT4, eurekanate-attachment enzyme; SUS1, sucrose synthase; NY2A_B736L, putative mannosyltransferase; AtGlgA, glycogen synthase; EcGlgA, glycogen synthase; PaGlgA, glycogen synthase; OsGBSSI, rice granule bound starch synthase; HvSSI, barley starch synthase I; WaaF, LPS heptosyltransferase II; WaaC, heptosyltransferase I; Vpar_0760, putative heptosyltransferase; FucT α-1,3-fucosyltransferase; OtsA, α,α-trehalose-phosphate synthase; Fut8, N-acetyl-d-glucosaminide -1,6-l-fucosyltransferase; NodZ, α-1,6-l-fucosyltransferase; MurG, UDP-GlcNAc: N-acetylmuramyl-(pentapeptide)-PP-C55 N-acetylglucosaminyltransferase; WaaA, CMP-β-KDO: α-3-deoxy-d-manno-2-octulosonic-acid (KDO) transferase; GP, glycogen phosphorylase; Gph1, glycogen phosphorylase; MalP, maltodextrin phosphorylase; SP, starch phosphorylase; NST, CMP-Neu: (LOS) β-galactosamide α-2,3-sialyltransferase; BGT, UDP-Glc: DNA β-glucosyltransferase; GumK, UDP-GlcA: (xanthan) α-Man-(1,3)-β-Glc-(1,4)- α-Glc-PP-polyisoprenyl β-1,2-glucuronosyltransferase; AGT, UDP-Glc: DNA α-glucosyltransferase; PmST1, CMP-NeuAc: α-2,3/2,6-sialyltransferase 1; ST, CMP-NeuAc: a-/β-galactoside α-2,3-sialyltransferase; Pst6-224, CMP-NeuAc: β-galactoside α-2,6-sialyltransferase (for further details, see www.cazy.org).