Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1985 Nov;82(21):7207–7211. doi: 10.1073/pnas.82.21.7207

A model for actin polymerization and the kinetic effects of ATP hydrolysis.

D Pantaloni, T L Hill, M F Carlier, E D Korn
PMCID: PMC390818  PMID: 3864156

Abstract

A model for actin polymerization is proposed in which the rate of elongation of actin filaments depends on whether adenosine 5'-triphosphate or adenosine 5'-diphosphate is bound to the two terminal subunits of the filament. This model accounts quantitatively for the experimental data on the kinetics of filament elongation and explains the effect of ATP hydrolysis on actin polymerization.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carlier M. F., Hill T. L., Chen Y. Interference of GTP hydrolysis in the mechanism of microtubule assembly: an experimental study. Proc Natl Acad Sci U S A. 1984 Feb;81(3):771–775. doi: 10.1073/pnas.81.3.771. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carlier M. F., Pantaloni D., Korn E. D. Evidence for an ATP cap at the ends of actin filaments and its regulation of the F-actin steady state. J Biol Chem. 1984 Aug 25;259(16):9983–9986. [PubMed] [Google Scholar]
  3. Carlier M. F., Pantaloni D., Korn E. D. Polymerization of ADP-actin and ATP-actin under sonication and characteristics of the ATP-actin equilibrium polymer. J Biol Chem. 1985 Jun 10;260(11):6565–6571. [PubMed] [Google Scholar]
  4. Chen Y. D., Hill T. L. Monte Carlo study of the GTP cap in a five-start helix model of a microtubule. Proc Natl Acad Sci U S A. 1985 Feb;82(4):1131–1135. doi: 10.1073/pnas.82.4.1131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chen Y., Hill T. L. Theoretical treatment of microtubules disappearing in solution. Proc Natl Acad Sci U S A. 1985 Jun;82(12):4127–4131. doi: 10.1073/pnas.82.12.4127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cooke R. The role of the bound nucleotide in the polymerization of actin. Biochemistry. 1975 Jul 15;14(14):3250–3256. doi: 10.1021/bi00685a035. [DOI] [PubMed] [Google Scholar]
  7. Cooper J. A., Buhle E. L., Jr, Walker S. B., Tsong T. Y., Pollard T. D. Kinetic evidence for a monomer activation step in actin polymerization. Biochemistry. 1983 Apr 26;22(9):2193–2202. doi: 10.1021/bi00278a021. [DOI] [PubMed] [Google Scholar]
  8. Frieden C., Goddette D. W. Polymerization of actin and actin-like systems: evaluation of the time course of polymerization in relation to the mechanism. Biochemistry. 1983 Dec 6;22(25):5836–5843. doi: 10.1021/bi00294a023. [DOI] [PubMed] [Google Scholar]
  9. Frieden C. Polymerization of actin: mechanism of the Mg2+-induced process at pH 8 and 20 degrees C. Proc Natl Acad Sci U S A. 1983 Nov;80(21):6513–6517. doi: 10.1073/pnas.80.21.6513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hill T. L., Chen Y. Phase changes at the end of a microtubule with a GTP cap. Proc Natl Acad Sci U S A. 1984 Sep;81(18):5772–5776. doi: 10.1073/pnas.81.18.5772. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hill T. L. Introductory analysis of the GTP-cap phase-change kinetics at the end of a microtubule. Proc Natl Acad Sci U S A. 1984 Nov;81(21):6728–6732. doi: 10.1073/pnas.81.21.6728. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hill T. L. Phase-change kinetics for a microtubule with two free ends. Proc Natl Acad Sci U S A. 1985 Jan;82(2):431–435. doi: 10.1073/pnas.82.2.431. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Korn E. D. Actin polymerization and its regulation by proteins from nonmuscle cells. Physiol Rev. 1982 Apr;62(2):672–737. doi: 10.1152/physrev.1982.62.2.672. [DOI] [PubMed] [Google Scholar]
  14. Kouyama T., Mihashi K. Fluorimetry study of N-(1-pyrenyl)iodoacetamide-labelled F-actin. Local structural change of actin protomer both on polymerization and on binding of heavy meromyosin. Eur J Biochem. 1981;114(1):33–38. [PubMed] [Google Scholar]
  15. Lal A. A., Korn E. D., Brenner S. L. Rate constants for actin polymerization in ATP determined using cross-linked actin trimers as nuclei. J Biol Chem. 1984 Jul 25;259(14):8794–8800. [PubMed] [Google Scholar]
  16. Mitchison T., Kirschner M. Dynamic instability of microtubule growth. Nature. 1984 Nov 15;312(5991):237–242. doi: 10.1038/312237a0. [DOI] [PubMed] [Google Scholar]
  17. Mitchison T., Kirschner M. Microtubule assembly nucleated by isolated centrosomes. Nature. 1984 Nov 15;312(5991):232–237. doi: 10.1038/312232a0. [DOI] [PubMed] [Google Scholar]
  18. OOSAWA F., KASAI M. A theory of linear and helical aggregations of macromolecules. J Mol Biol. 1962 Jan;4:10–21. doi: 10.1016/s0022-2836(62)80112-0. [DOI] [PubMed] [Google Scholar]
  19. Pantaloni D., Carlier M. F., Korn E. D. The interaction between ATP-actin and ADP-actin. A tentative model for actin polymerization. J Biol Chem. 1985 Jun 10;260(11):6572–6578. [PubMed] [Google Scholar]
  20. Pardee J. D., Spudich J. A. Mechanism of K+-induced actin assembly. J Cell Biol. 1982 Jun;93(3):648–654. doi: 10.1083/jcb.93.3.648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Pollard T. D., Weeds A. G. The rate constant for ATP hydrolysis by polymerized actin. FEBS Lett. 1984 May 7;170(1):94–98. doi: 10.1016/0014-5793(84)81376-9. [DOI] [PubMed] [Google Scholar]
  22. Tobacman L. S., Korn E. D. The kinetics of actin nucleation and polymerization. J Biol Chem. 1983 Mar 10;258(5):3207–3214. [PubMed] [Google Scholar]
  23. Weeds A. Actin-binding proteins--regulators of cell architecture and motility. Nature. 1982 Apr 29;296(5860):811–816. doi: 10.1038/296811a0. [DOI] [PubMed] [Google Scholar]
  24. Wegner A., Engel J. Kinetics of the cooperative association of actin to actin filaments. Biophys Chem. 1975 Jul;3(3):215–225. doi: 10.1016/0301-4622(75)80013-5. [DOI] [PubMed] [Google Scholar]
  25. Wegner A. Head to tail polymerization of actin. J Mol Biol. 1976 Nov;108(1):139–150. doi: 10.1016/s0022-2836(76)80100-3. [DOI] [PubMed] [Google Scholar]
  26. Wegner A., Savko P. Fragmentation of actin filaments. Biochemistry. 1982 Apr 13;21(8):1909–1913. doi: 10.1021/bi00537a032. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES