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. 1985 Dec;82(23):7810–7814. doi: 10.1073/pnas.82.23.7810

Loss of the normal N-ras allele in a mouse thymic lymphoma induced by a chemical carcinogen.

I Guerrero, A Villasante, V Corces, A Pellicer
PMCID: PMC390859  PMID: 3865197

Abstract

Young mice injected with the carcinogen N-nitroso-N-methylurea develop thymic lymphomas 2-4 months later. We previously have shown that these tumors frequently contain an activated N-ras gene that can transform rodent fibroblasts in vitro. We report here the intron/exon structure of such an activated N-ras gene and the sequence of its four coding exons. A single nucleotide change is responsible for the transforming alteration, a C----A transversion in the first base of codon 61. Through the use of synthetic oligonucleotides as hybridization probes, we show that this tumor lacks the normal allele of the N-ras gene. The implications of this finding for oncogene dominance are discussed.

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Selected References

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