Fig. 3.

mAY, but not IR, inhibits tumor angiogenesis and vessel stability, whereas IR enhances these inhibitory effects of mAY on vessels. (A) Animal tumor samples from Figure 2 were subjected to single staining (brown) of IHC for CD31, YKL-40 and SMa and double immunofluorescent staining for SMa (green)/CD31 (red) and fibrinogen (green)/CD31 (red). DAPI (blue) was used to stain nuclei. n = 6. Bars: 100 µm. (B) Quantification of CD31 vessel density and vessel diameter was shown from single immunohistochemical staining of CD31 in (A). Quantification of the ratio of fibrinogen versus CD31 from (A) was shown for vessel leakage. n = 6. *^,+ P <0.05 compared with mIgG and mAY, respectively.