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. 1985 Dec;82(24):8349–8353. doi: 10.1073/pnas.82.24.8349

Nonconsensus branch-site sequences in the in vitro splicing of transcripts of mutant rabbit beta-globin genes.

R A Padgett, M M Konarska, M Aebi, H Hornig, C Weissmann, P A Sharp
PMCID: PMC390913  PMID: 3866228

Abstract

Mutants of the rabbit beta-globin gene lacking the natural site of branch formation in the second intervening sequence have been analyzed for in vitro splicing activity. RNAs transcribed from these mutants were spliced, via lariat formation, at a reduced rate compared to wild-type RNA. The sites of branch formation were mapped by direct RNA analysis and primer-extension analysis. The sequences at the branch sites in the three mutants examined did not conform to the previously determined consensus sequence, nor were the 5' splice sites and branch sites complementary.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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