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. 1985 Dec;82(24):8374–8378. doi: 10.1073/pnas.82.24.8374

Epidermal growth factor regulates the expression of its own receptor.

A J Clark, S Ishii, N Richert, G T Merlino, I Pastan
PMCID: PMC390918  PMID: 3001700

Abstract

The epidermal growth factor (EGF) receptor gene is the cellular homolog of the avian erythroblastosis virus erbB oncogene. Control of EGF receptor expression determines cellular responsiveness to EGF and might play an important role in neoplastic development. Using RNA blot hybridization, we have found that exposure of human KB carcinoma cells to EGF results in elevated levels of EGF receptor mRNA. The phorbol ester 4 beta-phorbol 12-myristate 13-acetate also stimulates EGF receptor RNA accumulation. Immunoprecipitation of metabolically labeled (30 min) EGF receptor protein revealed that synthesis of new EGF receptor follows the increase in receptor RNA. Addition of cycloheximide together with EGF further enhances EGF receptor RNA accumulation. Results of nuclear runoff-transcription experiments suggest that the stimulatory effects of EGF and cycloheximide are most likely due to a posttranscriptional control mechanism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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