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. 1985 Dec;82(24):8404–8408. doi: 10.1073/pnas.82.24.8404

Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector.

G E Smith, G Ju, B L Ericson, J Moschera, H W Lahm, R Chizzonite, M D Summers
PMCID: PMC390924  PMID: 3878519

Abstract

A cDNA coding for human interleukin 2 (IL-2) was inserted into the genome of Autographa californica nuclear polyhedrosis virus adjacent to the polyhedrin promoter. Cells infected with recombinant virus produced high levels of Mr 15,500 IL-2 polypeptide, the majority of which was secreted into the culture medium during infection. The recombinant IL-2 was able to stimulate the growth of an IL-2-dependent cell line. The N-terminal amino acid sequence of the insect-derived IL-2 was identical to that of natural IL-2. Thus, a mammalian signal peptide was recognized and properly removed in insect cells.

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