Abstract
Six independent derivatives of the bz-m13 allele, which contains a 2.2-kilobase-pair defective Suppressor-mutator (dSpm) insertion at the bronze (bz) locus, have been isolated and analyzed. The derivatives were selected for alterations in the frequency and timing of somatic reversion; such derivatives have previously been analyzed genetically and designated "changes in state" by McClintock [McClintock, B. (1955) Carnegie Inst. Washington, Yearb. 54, 245-255]. All of the derivatives analyzed in the present study revert substantially later in development than the original insertion mutation and some show a very low frequency of reversion as well. All of the derivatives contain insertions at the same site as the parent bz-m13 allele. Deletions of 400-1300 base pairs were found in the dSpm elements in four of the six derivatives; the remaining derivatives could not be distinguished structurally from the original mutant allele. The results suggest that changes in the frequency and developmental timing of excision are attributable to alterations in the dSpm element. Furthermore, these data suggest that DNA sequences near the ends of the element are important for responding to the two transacting functions supplied by the transposition-competent Suppressor-mutator (Spm) element.
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- Benton W. D., Davis R. W. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. doi: 10.1126/science.322279. [DOI] [PubMed] [Google Scholar]
- Brink R. A., Williams E. Mutable R-navajo alleles of cyclic origin in maize. Genetics. 1973 Feb;73(2):273–296. doi: 10.1093/genetics/73.2.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Courage-Tebbe U., Döring H. P., Fedoroff N., Starlinger P. The controlling element Ds at the Shrunken locus in Zea mays: structure of the unstable sh-m5933 allele and several revertants. Cell. 1983 Sep;34(2):383–393. doi: 10.1016/0092-8674(83)90372-0. [DOI] [PubMed] [Google Scholar]
- Dooner H. K., Nelson O. E. Genetic control of UDPglucose:flavonol 3-O-glucosyltransferase in the endosperm of maize. Biochem Genet. 1977 Jun;15(5-6):509–519. doi: 10.1007/BF00520194. [DOI] [PubMed] [Google Scholar]
- Döring H. P., Starlinger P. Barbara McClintock's controlling elements: now at the DNA level. Cell. 1984 Dec;39(2 Pt 1):253–259. doi: 10.1016/0092-8674(84)90002-3. [DOI] [PubMed] [Google Scholar]
- Fedoroff N. V., Furtek D. B., Nelson O. E. Cloning of the bronze locus in maize by a simple and generalizable procedure using the transposable controlling element Activator (Ac). Proc Natl Acad Sci U S A. 1984 Jun;81(12):3825–3829. doi: 10.1073/pnas.81.12.3825. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fedoroff N., Mauvais J., Chaleff D. Molecular studies on mutations at the Shrunken locus in maize caused by the controlling element Ds. J Mol Appl Genet. 1983;2(1):11–29. [PubMed] [Google Scholar]
- Fedoroff N., Shure M., Kelly S., Johns M., Furtek D., Schiefelbein J., Nelson O. Isolation of Spm controlling elements from maize. Cold Spring Harb Symp Quant Biol. 1984;49:339–345. doi: 10.1101/sqb.1984.049.01.040. [DOI] [PubMed] [Google Scholar]
- Fedoroff N., Wessler S., Shure M. Isolation of the transposable maize controlling elements Ac and Ds. Cell. 1983 Nov;35(1):235–242. doi: 10.1016/0092-8674(83)90226-x. [DOI] [PubMed] [Google Scholar]
- Fincham J. R., Sastry G. R. Controlling elements in maize. Annu Rev Genet. 1974;8:15–50. doi: 10.1146/annurev.ge.08.120174.000311. [DOI] [PubMed] [Google Scholar]
- Frischauf A. M., Lehrach H., Poustka A., Murray N. Lambda replacement vectors carrying polylinker sequences. J Mol Biol. 1983 Nov 15;170(4):827–842. doi: 10.1016/s0022-2836(83)80190-9. [DOI] [PubMed] [Google Scholar]
- Gierl A., Schwarz-Sommer Z., Saedler H. Molecular interactions between the components of the En-I transposable element system of Zea mays. EMBO J. 1985 Mar;4(3):579–583. doi: 10.1002/j.1460-2075.1985.tb03669.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Klein A. S., Nelson O. E. Biochemical consequences of the insertion of a suppressor-mutator (Spm) receptor at the bronze-1 locus in maize. Proc Natl Acad Sci U S A. 1983 Dec;80(24):7591–7595. doi: 10.1073/pnas.80.24.7591. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Larson R. L., Coe E. H., Jr Gene-dependent flavonoid glucosyltransferase in maize. Biochem Genet. 1977 Feb;15(1-2):153–156. doi: 10.1007/BF00484558. [DOI] [PubMed] [Google Scholar]
- NUFFER M. G. Mutation studies at the A. locus in maize. I. A mutable allele controlled by Dt. Genetics. 1961 Jun;46:625–640. doi: 10.1093/genetics/46.6.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nelson O. E., Klein A. S. Characterization of an spm-controlled bronze-mutable allele in maize. Genetics. 1984 Apr;106(4):769–779. doi: 10.1093/genetics/106.4.769. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pereira A., Schwarz-Sommer Z., Gierl A., Bertram I., Peterson P. A., Saedler H. Genetic and molecular analysis of the Enhancer (En) transposable element system of Zea mays. EMBO J. 1985 Jan;4(1):17–23. doi: 10.1002/j.1460-2075.1985.tb02311.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Peterson P A. Mutable a(1) of the En System in Maize. Genetics. 1961 Jul;46(7):759–771. doi: 10.1093/genetics/46.7.759. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schwarz-Sommer Z., Gierl A., Klösgen R. B., Wienand U., Peterson P. A., Saedler H. The Spm (En) transposable element controls the excision of a 2-kb DNA insert at the wx allele of Zea mays. EMBO J. 1984 May;3(5):1021–1028. doi: 10.1002/j.1460-2075.1984.tb01922.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shure M., Wessler S., Fedoroff N. Molecular identification and isolation of the Waxy locus in maize. Cell. 1983 Nov;35(1):225–233. doi: 10.1016/0092-8674(83)90225-8. [DOI] [PubMed] [Google Scholar]
- Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
- Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]