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Overlays of the downfield region of the 2D 1H-1H TOCSY spectra of ZF4 and CP1 at 15 ºC and HR1 at 10 ºC incubated with (red) without and 1 (gray). Boxes indicate crosspeaks between histidine side chain methine protons H2 and H5 for all His residues in each peptide. Notable upfield shifts of His proton resonances are observed in all peptides in the presence of 1 (red boxes) as compared to resonances of the free peptides (gray boxes).
