Abstract
Determination of the amino acid sequence of bovine parathyroid hormone has led to the synthesis of a tetratriacontapeptide corresponding to the amino-terminal 1-34 residues of the native molecule. The specific biological effects of this synthetic peptide on bone and kidney are qualitatively identical to those of the native hormone in classical bioassays in vivo and in several systems in vitro. Potency of the synthetic peptide equals or exceeds that of a biologically active fragment of comparable size isolated from the native hormone; the synthetic and natural peptides show complete immunological cross-reactivity. Thus, essential requirements for the physiological actions of the peptide on both skeletal and renal tissue are contained within the 34 amino-terminal amino acids. The potency of the synthetic peptide, relative to that of the native (84-amino acid) polypeptide, is greater in vitro than in vivo; this suggests that the carboxyl terminal two-thirds of the native hormone may protect the circulating polypeptide from rapid metabolic degradation.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- AURBACH G. D. The influence of gelatin in the assay of purified parathyroid extracts. Endocrinology. 1959 Feb;64(2):296–298. doi: 10.1210/endo-64-2-296. [DOI] [PubMed] [Google Scholar]
- Brewer H. B., Jr, Ronan R. Bovine parathyroid hormone: amino acid sequence. Proc Natl Acad Sci U S A. 1970 Dec;67(4):1862–1869. doi: 10.1073/pnas.67.4.1862. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chase L. R., Aurbach G. D. Parathyroid function and the renal excretion of 3'5'-adenylic acid. Proc Natl Acad Sci U S A. 1967 Aug;58(2):518–525. doi: 10.1073/pnas.58.2.518. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chase L. R., Aurbach G. D. The effect of parathyroid hormone on the concentration of adenosine 3',5'-monophosphate in skeletal tissue in vitro. J Biol Chem. 1970 Apr 10;245(7):1520–1526. [PubMed] [Google Scholar]
- Kaiser E., Colescott R. L., Bossinger C. D., Cook P. I. Color test for detection of free terminal amino groups in the solid-phase synthesis of peptides. Anal Biochem. 1970 Apr;34(2):595–598. doi: 10.1016/0003-2697(70)90146-6. [DOI] [PubMed] [Google Scholar]
- Marcus R., Aurbach G. D. Bioassay of parathyroid hormone in vitro with a stable preparation of adenyl cyclase from rat kidney. Endocrinology. 1969 Nov;85(5):801–810. doi: 10.1210/endo-85-5-801. [DOI] [PubMed] [Google Scholar]
- Merrifield R. B. Solid-phase peptide synthesis. Adv Enzymol Relat Areas Mol Biol. 1969;32:221–296. doi: 10.1002/9780470122778.ch6. [DOI] [PubMed] [Google Scholar]
- Ontjes D. A., Anfinsen C. B. Solid phase synthesis of a 42-residue fragment of staphylococcal nuclease: properties of a semisynthetic enzyme. Proc Natl Acad Sci U S A. 1969 Oct;64(2):428–435. doi: 10.1073/pnas.64.2.428. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Potts J. T., Jr, Aurbach G. D., Sherwood L. M. Parathyroid hormone: chemical properties and structural requirements for biological and immunological activity. Recent Prog Horm Res. 1966;22:101–151. doi: 10.1016/b978-1-4831-9825-5.50006-3. [DOI] [PubMed] [Google Scholar]
- Potts J. T., Jr, Niall H. D., Keutmann H. T., Brewer H. B., Jr, Deftos L. J. The amino acid sequence of porcine thyrocalcitonin. Proc Natl Acad Sci U S A. 1968 Apr;59(4):1321–1328. doi: 10.1073/pnas.59.4.1321. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Takashima H., Du Vigneaud V., Merrifield R. B. The synthesis of deamino-oxytocin by the solid phase method. J Am Chem Soc. 1968 Feb 28;90(5):1323–1325. doi: 10.1021/ja01007a038. [DOI] [PubMed] [Google Scholar]