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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1971 Jan;68(1):87–91. doi: 10.1073/pnas.68.1.87

Fatty Acid Synthetase Activity in Mycobacterium phlei: Regulation by Polysaccharides

M Ilton 1, A W Jevans 1, E D McCarthy 1, D Vance 1, H B White III 1, Konrad Bloch 1
PMCID: PMC391108  PMID: 5276305

Abstract

The multienzyme complex from Mycobacterium phlei which catalyzes the synthesis of long chain fatty acids from acetyl-CoA and malonyl-CoA requires a heat-stable fraction (stimulating factor, SF) for activity. Fractionation of heat-treated M. phlei extracts affords two stimulatory subfractions, one of which (SF2) can be replaced by FMN. The other (SF1) is further separable into 3 polysaccharides (PSI, PSII, and PSIII). PSI contains about 95% 3-O-methylmannose and 5% mannose; the sugar composition of PSII and PSIII is about 55% 6-O-methylglucose and 45% glucose for both. Each of the three purified polysaccharides, in combination with FMN, substitutes for the crude stimulating factor. The polysaccharides exert their effect on the fatty acid synthetase by lowering the Km for acetyl-CoA about 50-fold.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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