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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1971 Jan;68(1):240–243. doi: 10.1073/pnas.68.1.240

A Thrombin-Sensitive Protein of Human Platelet Membranes

Nancy Lewis Baenziger 1,2,*, G N Brodie 1,2,†, Philip W Majerus 1,2,‡
PMCID: PMC391203  PMID: 5276296

Abstract

The action of thrombin on intact human platelets has been studied with the aid of polyacrylamide gel electrophoresis in sodium dodecylsulfate. A single major membrane protein band with a molecular weight of 190,000 disappears after thrombin treatment, while a new membrane protein with a molecular weight of 107,000 appears. This may represent hydrolysis of the thrombin-sensitive protein. When platelets are disrupted or when the thrombin-sensitive protein is solubilized from membranes prior to thrombin treatment, no hydrolysis occurs. The effect of thrombin on the platelet membrane protein is complete within 2 min which suggests that hydrolysis of this membrane protein may trigger the physiological effects of thrombin on platelets.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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