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. 1983 Nov;80(21):6586–6590. doi: 10.1073/pnas.80.21.6586

Localization of cellular retinol-binding protein in several rat tissues.

S B Porter, L D Fraker, F Chytil, D E Ong
PMCID: PMC391214  PMID: 6356129

Abstract

The distribution of cellular retinol-binding protein (CRBP) in rat liver, ileum, and epididymis was examined by the peroxidase-antiperoxidase immunolocalization technique. Positive cytoplasmic staining was seen in the liver when antiserum prepared against purified CRBP was used but not when antiserum absorbed with purified CRBP was used. Ileal mucosa, a tissue that contains no detectable CRBP, showed no positive staining. The epididymis showed strong positive staining in the caput but not in the cauda. Staining was present in principal and basal cells but not in peritubular or interstitial cells. Radioimmunoassay revealed that the CRBP within the caput epididymidis was localized in the initial segment and proximal region, areas known to be involved in the synthesis and secretion of factors necessary for sperm maturation. The results demonstrate that the expression of CRBP may vary within the same cell type, as well as between different cell types within the same tissue.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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