Abstract
Cell sorting among prespore cells and prestalk cells in Dictyostelium discoideum was studied by using fluorochrome-labeled cells in an in vitro assay. Labeled prestalk cells first formed randomly mixed aggregates with unlabeled prespore cells. Then cells began to sort out from each other. About 3-4 hr later, prestalk cells became clustered at one pole of the aggregate. Aggregates deposited on an agar surface underwent morphogenesis and formed migrating slugs within 3 hr. The addition of Fab fragments directed against a cell-surface glycoprotein of Mr 150,000 (gp 150) to the cell mixture completely inhibited the cell-sorting phenomenon. Morphogenesis of such aggregates on agar was also delayed by 5 hr. However, inclusion of Fab fragments directed against the endogenous lectins, the contact site A glycoprotein, or vegetative cells had no detectable effect on cell sorting or morphogenesis of these reconstituted aggregates.
Full text
PDF




Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bertolotti R., Rutishauser U., Edelman G. M. A cell surface molecule involved in aggregation of embryonic liver cells. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4831–4835. doi: 10.1073/pnas.77.8.4831. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Beug H., Gerisch G., Kempff S., Riedel V., Cremer G. Specific inhibition of cell contact formation in Dictyostelium by univalent antibodies. Exp Cell Res. 1970 Nov;63(1):147–158. doi: 10.1016/0014-4827(70)90343-5. [DOI] [PubMed] [Google Scholar]
- Bonner J. T. EVIDENCE FOR THE SORTING OUT OF CELLS IN THE DEVELOPMENT OF THE CELLULAR SLIME MOLDS. Proc Natl Acad Sci U S A. 1959 Mar;45(3):379–384. doi: 10.1073/pnas.45.3.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Feinberg A. P., Springer W. R., Barondes S. H. Segregation of pre-stalk and pre-spore cells of Dictyostelium discoideum: observations consistent with selective cell cohesion. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3977–3981. doi: 10.1073/pnas.76.8.3977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Forman D., Garrod D. R. Pattern formation in Dictyostelium discoideum. II. Differentiation and pattern formation in non-polar aggregates. J Embryol Exp Morphol. 1977 Aug;40:229–243. [PubMed] [Google Scholar]
- Geltosky J. E., Birdwell C. R., Weseman J., Lerner R. A. A glycoprotein involved in aggregation of D. discoideum is distributed on the cell surface in a nonrandom fashion favoring cell junctions. Cell. 1980 Sep;21(2):339–345. doi: 10.1016/0092-8674(80)90470-5. [DOI] [PubMed] [Google Scholar]
- Geltosky J. E., Siu C. H., Lerner R. A. Glycoproteins of the plasma membrane of Dictyostelium discoideum during development. Cell. 1976 Jul;8(3):391–396. doi: 10.1016/0092-8674(76)90151-3. [DOI] [PubMed] [Google Scholar]
- Geltosky J. E., Weseman J., Bakke A., Lerner R. A. Identification of a cell surface glycoprotein involved in cell aggregation in D. discoideum. Cell. 1979 Oct;18(2):391–398. doi: 10.1016/0092-8674(79)90058-8. [DOI] [PubMed] [Google Scholar]
- Gerisch G. Cell aggregation and differentiation in Dictyostelium. Curr Top Dev Biol. 1968;3:157–197. doi: 10.1016/s0070-2153(08)60354-3. [DOI] [PubMed] [Google Scholar]
- Hyafil F., Morello D., Babinet C., Jacob F. A cell surface glycoprotein involved in the compaction of embryonal carcinoma cells and cleavage stage embryos. Cell. 1980 Oct;21(3):927–934. doi: 10.1016/0092-8674(80)90456-0. [DOI] [PubMed] [Google Scholar]
- Loomis W. F. Biochemistry of Aggregation in Dictyostelium. A review. Dev Biol. 1979 May;70(1):1–12. doi: 10.1016/0012-1606(79)90002-2. [DOI] [PubMed] [Google Scholar]
- MacWilliams H. K., Bonner J. T. The prestalk-prespore pattern in cellular slime molds. Differentiation. 1979;14(1-2):1–22. doi: 10.1111/j.1432-0436.1979.tb01006.x. [DOI] [PubMed] [Google Scholar]
- Müller K., Gerisch G. A specific glycoprotein as the target site of adhesion blocking Fab in aggregating Dictyostelium cells. Nature. 1978 Aug 3;274(5670):445–449. doi: 10.1038/274445a0. [DOI] [PubMed] [Google Scholar]
- PUTNAM F. W., TAN M., LYNN L. T., EASLEY C. W., MIGITA S. The cleavage of rabbit gamma-globulin by papain. J Biol Chem. 1962 Mar;237:717–726. [PubMed] [Google Scholar]
- Ray J., Shinnick T., Lerner R. A mutation altering the function of a carbohydrate binding protein blocks cell-cell cohesion in developing Dictyostelium discoideum. Nature. 1979 May 17;279(5710):215–221. doi: 10.1038/279215a0. [DOI] [PubMed] [Google Scholar]
- Rosen S. D., Kafka J. A., Simpson D. L., Barondes S. H. Developmentally regulated, carbohydrate-binding protein in Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2554–2557. doi: 10.1073/pnas.70.9.2554. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rutishauser U., Thiery J. P., Brackenbury R., Sela B. A., Edelman G. M. Mechanisms of adhesion among cells from neural tissues of the chick embryo. Proc Natl Acad Sci U S A. 1976 Feb;73(2):577–581. doi: 10.1073/pnas.73.2.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Saxe C. L., 3rd, Sussman M. Induction of stage-specific cell cohesion in D. discoideum by a plasma-membrane-associated moiety reactive with wheat germ agglutinin. Cell. 1982 Jul;29(3):755–759. doi: 10.1016/0092-8674(82)90437-8. [DOI] [PubMed] [Google Scholar]
- Siu C. H., Lerner R. A., Ma G., Firtel R. A., Loomis W. F. Developmentally regulated proteins of the plasma membrane of Dictyostelium discoideum. The carbohydrate-binding protein. J Mol Biol. 1976 Jan 15;100(2):157–178. doi: 10.1016/s0022-2836(76)80146-5. [DOI] [PubMed] [Google Scholar]
- Springer W. R., Barondes S. H. Cell adhesion molecules: detection with univalent second antibody. J Cell Biol. 1980 Dec;87(3 Pt 1):703–707. doi: 10.1083/jcb.87.3.703. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Springer W. R., Barondes S. H. Direct measurement of species-specific cohesion in cellular slime molds. J Cell Biol. 1978 Sep;78(3):937–942. doi: 10.1083/jcb.78.3.937. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Steinberg M. S. Adhesion-guided multicellular assembly: a commentary upon the postulates, real and imagined, of the differential adhesion hypothesis, with special attention to computer simulations of cell sorting. J Theor Biol. 1975 Dec;55(2):431–443. doi: 10.1016/s0022-5193(75)80091-9. [DOI] [PubMed] [Google Scholar]
- Steinemann C., Parish R. W. Evidence that a developmentally regulated glycoprotein is target of adhesion-blocking Fab in reaggregating Dictyostelium. Nature. 1980 Aug 7;286(5773):621–623. doi: 10.1038/286621a0. [DOI] [PubMed] [Google Scholar]
- Tasaka M., Takeuchi I. Sorting out behaviour of disaggregated cells in the absence of morphogenesis in Dictyostelium discoideum. J Embryol Exp Morphol. 1979 Jan;49:89–102. [PubMed] [Google Scholar]
- Wilcox D. K., Sussman M. Serologically distinguishable alterations in the molecular specificity of cell cohesion during morphogenesis in Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1981 Jan;78(1):358–362. doi: 10.1073/pnas.78.1.358. [DOI] [PMC free article] [PubMed] [Google Scholar]