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. 1985 Dec;82(23):8014–8018. doi: 10.1073/pnas.82.23.8014

Expression of a functional human insulin receptor from a cloned cDNA in Chinese hamster ovary cells.

Y Ebina, M Edery, L Ellis, D Standring, J Beaudoin, R A Roth, W J Rutter
PMCID: PMC391432  PMID: 3906655

Abstract

We have placed human insulin receptor cDNA into a vector under the control of the simian virus 40 (SV40) early promoter and tested its function by transient expression in microinjected Xenopus oocytes and by expression in stably transformed CHO cells. The precursor and the alpha and beta subunits of the receptor were detected by immunoprecipitation from extracts of these cells. The human insulin receptor expressed in CHO cells specifically binds 125I-labeled insulin but not insulin-like growth factor I, displays insulin-stimulated autophosphorylation of the beta subunit, and mediates insulin-stimulated 2-deoxyglucose uptake. We conclude that the human insulin receptor is synthesized, processed normally, and functional in this heterologous cell system.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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