Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1985 Dec;82(23):8119–8123. doi: 10.1073/pnas.82.23.8119

Transposase titration in Drosophila melanogaster: a model of cytotype in the P-M system of hybrid dysgenesis.

M J Simmons, L M Bucholz
PMCID: PMC391454  PMID: 2999793

Abstract

In the P-M system of hybrid dysgenesis of Drosophila melanogaster, some M strains possess chromosomal P elements. The chromosomes from one of these pseudo-M strains reduced the instability of a P element-insertion mutation of the singed bristle locus. We suggest that this reduction is an indication of competition for a transposase that binds to the P elements on the chromosomes from the pseudo-M strain as well as to the P elements at the singed locus; the pseudo-M strain's P elements might therefore be said to titrate the transposase, reducing its availability to interact with the P elements at the singed locus. We hypothesize that a similar mechanism regulates the movement of P elements in the P strains of D. melanogaster, although in this case we propose that the titrating elements are extrachromosomal and that they are generated by the action of the transposase itself.

Full text

PDF
8119

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bingham P. M., Kidwell M. G., Rubin G. M. The molecular basis of P-M hybrid dysgenesis: the role of the P element, a P-strain-specific transposon family. Cell. 1982 Jul;29(3):995–1004. doi: 10.1016/0092-8674(82)90463-9. [DOI] [PubMed] [Google Scholar]
  2. Bingham P. M., Levis R., Rubin G. M. Cloning of DNA sequences from the white locus of D. melanogaster by a novel and general method. Cell. 1981 Sep;25(3):693–704. doi: 10.1016/0092-8674(81)90176-8. [DOI] [PubMed] [Google Scholar]
  3. Bregliano J. C., Picard G., Bucheton A., Pelisson A., Lavige J. M., L'Heritier P. Hybrid dysgenesis in Drosophila melanogaster. Science. 1980 Feb 8;207(4431):606–611. doi: 10.1126/science.6766221. [DOI] [PubMed] [Google Scholar]
  4. Bucheton A. Non-Mendelian female sterility in Drosophila melanogaster: influence of aging and thermic treatments. III. Cumulative effects induced by these factors. Genetics. 1979 Sep;93(1):131–142. doi: 10.1093/genetics/93.1.131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bucheton A., Paro R., Sang H. M., Pelisson A., Finnegan D. J. The molecular basis of I-R hybrid dysgenesis in Drosophila melanogaster: identification, cloning, and properties of the I factor. Cell. 1984 Aug;38(1):153–163. doi: 10.1016/0092-8674(84)90536-1. [DOI] [PubMed] [Google Scholar]
  6. Engels W. R. A trans-acting product needed for P factor transposition in Drosophila. Science. 1984 Dec 7;226(4679):1194–1196. doi: 10.1126/science.6095450. [DOI] [PubMed] [Google Scholar]
  7. Engels W. R. Extrachromosomal control of mutability in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1979 Aug;76(8):4011–4015. doi: 10.1073/pnas.76.8.4011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Engels W. R. Hybrid dysgenesis in Drosophila and the Stochastic loss hypothesis. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):561–565. doi: 10.1101/sqb.1981.045.01.072. [DOI] [PubMed] [Google Scholar]
  9. Engels W. R., Preston C. R. Formation of chromosome rearrangements by P factors in Drosophila. Genetics. 1984 Aug;107(4):657–678. doi: 10.1093/genetics/107.4.657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Engels W. R. The P family of transposable elements in Drosophila. Annu Rev Genet. 1983;17:315–344. doi: 10.1146/annurev.ge.17.120183.001531. [DOI] [PubMed] [Google Scholar]
  11. Engels W. R. The estimation of mutation rates when premeiotic events are involved. Environ Mutagen. 1979;1(1):37–43. doi: 10.1002/em.2860010110. [DOI] [PubMed] [Google Scholar]
  12. Flavell A. J., Ish-Horowicz D. Extrachromosomal circular copies of the eukaryotic transposable element copia in cultured Drosophila cells. Nature. 1981 Aug 13;292(5824):591–595. doi: 10.1038/292591a0. [DOI] [PubMed] [Google Scholar]
  13. Flavell A. J., Ish-Horowicz D. The origin of extrachromosomal circular copia elements. Cell. 1983 Sep;34(2):415–419. doi: 10.1016/0092-8674(83)90375-6. [DOI] [PubMed] [Google Scholar]
  14. Karess R. E., Rubin G. M. Analysis of P transposable element functions in Drosophila. Cell. 1984 Aug;38(1):135–146. doi: 10.1016/0092-8674(84)90534-8. [DOI] [PubMed] [Google Scholar]
  15. Kidwell M. G. Evolution of hybrid dysgenesis determinants in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1983 Mar;80(6):1655–1659. doi: 10.1073/pnas.80.6.1655. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kidwell M. G., Kidwell J. F., Sved J. A. Hybrid Dysgenesis in DROSOPHILA MELANOGASTER: A Syndrome of Aberrant Traits Including Mutation, Sterility and Male Recombination. Genetics. 1977 Aug;86(4):813–833. doi: 10.1093/genetics/86.4.813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. O'Hare K., Rubin G. M. Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genome. Cell. 1983 Aug;34(1):25–35. doi: 10.1016/0092-8674(83)90133-2. [DOI] [PubMed] [Google Scholar]
  18. Picard G. Non-mendelian female sterility in Drosophila melanogaster: hereditary transmission of I factor. Genetics. 1976 May;83(1):107–123. doi: 10.1093/genetics/83.1.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ronsseray S., Anxolabéhère D., Périquet G. Hybrid dysgenesis in Drosophila melanogaster: influence of temperature on cytotype determination in the P-M system. Mol Gen Genet. 1984;196(1):17–23. doi: 10.1007/BF00334086. [DOI] [PubMed] [Google Scholar]
  20. Ruan K., Emmons S. W. Extrachromosomal copies of transposon Tc1 in the nematode Caenorhabditis elegans. Proc Natl Acad Sci U S A. 1984 Jul;81(13):4018–4022. doi: 10.1073/pnas.81.13.4018. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rubin G. M., Kidwell M. G., Bingham P. M. The molecular basis of P-M hybrid dysgenesis: the nature of induced mutations. Cell. 1982 Jul;29(3):987–994. doi: 10.1016/0092-8674(82)90462-7. [DOI] [PubMed] [Google Scholar]
  22. Rubin G. M., Spradling A. C. Genetic transformation of Drosophila with transposable element vectors. Science. 1982 Oct 22;218(4570):348–353. doi: 10.1126/science.6289436. [DOI] [PubMed] [Google Scholar]
  23. Simmons M. J., Raymond J. D., Culbert T. P., Laverty T. R. Analysis of dysgenesis-induced lethal mutations on the X chromosome of a Q strain of Drosophila melanogaster. Genetics. 1984 May;107(1):49–63. doi: 10.1093/genetics/107.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Spradling A. C., Rubin G. M. Transposition of cloned P elements into Drosophila germ line chromosomes. Science. 1982 Oct 22;218(4570):341–347. doi: 10.1126/science.6289435. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES