Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1985 Dec;82(23):8193–8197. doi: 10.1073/pnas.82.23.8193

Antigen-induced increase in protein kinase C activity in plasma membrane of mast cells.

J R White, D H Pluznik, K Ishizaka, T Ishizaka
PMCID: PMC391469  PMID: 2415983

Abstract

Bridging of cell-bound IgE antibody molecules on colony-stimulating factor-dependent mouse mast cell line (PT-18) cells by multivalent antigen induces phospholipid methylation, a transient rise in intracellular cAMP, intracellular mobilization and uptake of Ca2+, and formation of diacylglycerol followed by histamine release. Exposure of the sensitized cells to antigen also induces a substantial increase in protein kinase C activity in the plasma membrane, which is accompanied by a slight decrease in the enzyme in cytosol. Protein kinase C activity in the membrane fraction reached maximum within 30 sec after antigen challenge and then gradually declined. The increase of the enzyme activity in the membrane could not be explained by a shift of the enzyme from cytosol, and it suggested that bridging of IgE-receptor may induce a modulation of existing enzyme to a state of higher catalytic activity. Phorbol 12-myristate 13-acetate also induced a rapid but persistent increase in protein kinase C activity in the membrane fraction of mast cells. However, the increase in the enzyme activity in the membrane was accompanied by a marked decrease in the enzyme in cytosol.

Full text

PDF
8193

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Beaven M. A., Moore J. P., Smith G. A., Hesketh T. R., Metcalfe J. C. The calcium signal and phosphatidylinositol breakdown in 2H3 cells. J Biol Chem. 1984 Jun 10;259(11):7137–7142. [PubMed] [Google Scholar]
  3. Foreman J. C., Hallett M. B., Mongar J. L. The relationship between histamine secretion and 45calcium uptake by mast cells. J Physiol. 1977 Sep;271(1):193–214. doi: 10.1113/jphysiol.1977.sp011996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hamilton T. A., Becton D. L., Somers S. D., Gray P. W., Adams D. O. Interferon-gamma modulates protein kinase C activity in murine peritoneal macrophages. J Biol Chem. 1985 Feb 10;260(3):1378–1381. [PubMed] [Google Scholar]
  5. Hong S. L., Deykin D. The activation of phosphatidylinositol-hydrolyzing phospholipase A2 during prostaglandin synthesis in transformed mouse BALB/3T3 cells. J Biol Chem. 1981 May 25;256(10):5215–5219. [PubMed] [Google Scholar]
  6. Ishizaka T., Conrad D. H., Huff T. F., Metcalfe D. D., Stevens R. L., Lewis R. A. Unique features of human basophilic granulocytes developed in in vitro culture. Int Arch Allergy Appl Immunol. 1985;77(1-2):137–143. doi: 10.1159/000233768. [DOI] [PubMed] [Google Scholar]
  7. Ishizaka T., Foreman J. C., Sterk A. R., Ishizaka K. Induction of calcium flux across the rat mast cell membrane by bridging IgE receptors. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5858–5862. doi: 10.1073/pnas.76.11.5858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ishizaka T., Hirata F., Ishizaka K., Axelrod J. Stimulation of phospholipid methylation, Ca2+ influx, and histamine release by bridging of IgE receptors on rat mast cells. Proc Natl Acad Sci U S A. 1980 Apr;77(4):1903–1906. doi: 10.1073/pnas.77.4.1903. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ishizaka T., Hirata F., Sterk A. R., Ishizaka K., Axelrod J. A. Bridging of IgE receptors activates phospholipid methylation and adenylate cyclase in mast cell plasma membranes. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6812–6816. doi: 10.1073/pnas.78.11.6812. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ishizaka T., Ishizaka K. Biology of immunoglobulin E. Molecular basis of reaginic hypersensitivity. Prog Allergy. 1975;19:60–121. [PubMed] [Google Scholar]
  11. Ishizaka T., Sterk A. R., Daeron M., Becker E. L., Ishizaka K. Biochemical analysis of desensitization of mouse mast cells. J Immunol. 1985 Jul;135(1):492–501. [PubMed] [Google Scholar]
  12. Ishizuka Y., Nozawa Y. Concerted stimulation of PI-turnover, Ca2+-influx and histamine release in antigen-activated rat mast cells. Biochem Biophys Res Commun. 1983 Dec 28;117(3):710–717. doi: 10.1016/0006-291x(83)91655-8. [DOI] [PubMed] [Google Scholar]
  13. Kennerly D. A., Sullivan T. J., Sylwester P., Parker C. W. Diacylglycerol metabolism in mast cells: a potential role in membrane fusion and arachidonic acid release. J Exp Med. 1979 Oct 1;150(4):1039–1044. doi: 10.1084/jem.150.4.1039. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kishimoto A., Takai Y., Mori T., Kikkawa U., Nishizuka Y. Activation of calcium and phospholipid-dependent protein kinase by diacylglycerol, its possible relation to phosphatidylinositol turnover. J Biol Chem. 1980 Mar 25;255(6):2273–2276. [PubMed] [Google Scholar]
  15. Kraft A. S., Anderson W. B., Cooper H. L., Sando J. J. Decrease in cytosolic calcium/phospholipid-dependent protein kinase activity following phorbol ester treatment of EL4 thymoma cells. J Biol Chem. 1982 Nov 25;257(22):13193–13196. [PubMed] [Google Scholar]
  16. Kraft A. S., Anderson W. B. Phorbol esters increase the amount of Ca2+, phospholipid-dependent protein kinase associated with plasma membrane. Nature. 1983 Feb 17;301(5901):621–623. doi: 10.1038/301621a0. [DOI] [PubMed] [Google Scholar]
  17. Liu F. T., Bohn J. W., Ferry E. L., Yamamoto H., Molinaro C. A., Sherman L. A., Klinman N. R., Katz D. H. Monoclonal dinitrophenyl-specific murine IgE antibody: preparation, isolation, and characterization. J Immunol. 1980 Jun;124(6):2728–2737. [PubMed] [Google Scholar]
  18. Nabel G., Galli S. J., Dvorak A. M., Dvorak H. F., Cantor H. Inducer T lymphocytes synthesize a factor that stimulates proliferation of cloned mast cells. Nature. 1981 May 28;291(5813):332–334. doi: 10.1038/291332a0. [DOI] [PubMed] [Google Scholar]
  19. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  20. Siraganian R. P. An automated continuous-flow system for the extraction and fluorometric analysis of histamine. Anal Biochem. 1974 Feb;57(2):383–394. doi: 10.1016/0003-2697(74)90093-1. [DOI] [PubMed] [Google Scholar]
  21. Tsien R. Y., Pozzan T., Rink T. J. Calcium homeostasis in intact lymphocytes: cytoplasmic free calcium monitored with a new, intracellularly trapped fluorescent indicator. J Cell Biol. 1982 Aug;94(2):325–334. doi: 10.1083/jcb.94.2.325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. White J. R., Huang C. K., Hill J. M., Jr, Naccache P. H., Becker E. L., Sha'afi R. I. Effect of phorbol 12-myristate 13-acetate and its analogue 4 alpha-phorbol 12,13-didecanoate on protein phosphorylation and lysosomal enzyme release in rabbit neutrophils. J Biol Chem. 1984 Jul 10;259(13):8605–8611. [PubMed] [Google Scholar]
  23. White J. R., Ishizaka T., Ishizaka K., Sha'afi R. Direct demonstration of increased intracellular concentration of free calcium as measured by quin-2 in stimulated rat peritoneal mast cell. Proc Natl Acad Sci U S A. 1984 Jul;81(13):3978–3982. doi: 10.1073/pnas.81.13.3978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Woodin A. M., Wieneke A. A. The cation-sensitive phosphatases of the leucocyte cell membrane. Biochem Biophys Res Commun. 1968 Nov 25;33(4):558–562. doi: 10.1016/0006-291x(68)90331-8. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES