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. 1984 Aug;81(16):5160–5164. doi: 10.1073/pnas.81.16.5160

Receptors for human gamma interferon: binding and crosslinking of 125I-labeled recombinant human gamma interferon to receptors on WISH cells.

F H Sarkar, S L Gupta
PMCID: PMC391657  PMID: 6089181

Abstract

Purified recombinant human gamma interferon (HuIFN-gamma), labeled with 125I (125I-HuIFN-gamma), was used to study receptors for HuIFN-gamma on human WISH cells. 125I-HuIFN-gamma was bound to WISH cells, and this binding was displaced by unlabeled HuIFN-gamma but not by unlabeled recombinant HuIFN-alpha 2 or [Ser17]HuIFN-beta (HuIFN-beta with serine substituted for cysteine at position 17), indicating the presence of specific binding sites for HuIFN-gamma. The cell-bound 125I-HuIFN-gamma was crosslinked with disuccinimidyl suberate or ethylene glycol bis(succinimidyl succinate), which yielded a complex of Mr approximately 105,000 +/- 5000 as analyzed by NaDodSO4/PAGE. The formation of this complex was prevented by preincubation of cells with unlabeled HuIFN-gamma but not with HuIFN-alpha 2 or [Ser17]HuIFN-beta, indicating that HuIFN-gamma binds to a specific receptor molecule and that HuIFN-alpha 2 or HuIFN-beta do not interact with this receptor. Experiments were carried out with 125I-labeled recombinant [Ser17]HuIFN-beta (125I-[Ser17]HuIFN-beta) to verify this conclusion. Binding and crosslinking of 125I-[Ser17]HuIFN-beta to human WISH cells and Daudi cells yielded a complex of Mr approximately 150,000 similar to that obtained with 125I-HuIFN-alpha 2 as described earlier. The formation of this Mr 150,000 complex with 125I-[Ser17]HuIFN-beta was displaced by unlabeled [Ser17]HuIFN-beta and by HuIFN-alpha 2 but not by HuIFN-gamma, indicating that [Ser17]HuIFN-beta binds to the same receptor as does HuIFN-alpha 2, identified earlier, and that HuIFN-gamma does not compete with 125I-[Ser17]HuIFN-beta for this receptor. We conclude that HuIFN-gamma interacts with specific receptors that are distinctly different from the receptors recognized by HuIFN-alpha and HuIFN-beta.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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