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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1984 Sep;81(17):5399–5402. doi: 10.1073/pnas.81.17.5399

Synthesis and biological activity of human inhibin-like peptide-(1-31).

D Yamashiro, C H Li, K Ramasharma, M R Sairam
PMCID: PMC391711  PMID: 6433350

Abstract

A peptide containing 31 amino acids with inhibin-like activity has been synthesized by the solid-phase method. The synthetic product was shown to be homogeneous in high-performance liquid chromatography and paper electrophoresis. In the mouse pituitary incubation system, the follitropin-suppressing activity of the synthetic peptide was comparable to that of the natural product. It was also active in suppressing lutropin-releasing hormone-induced follitropin release in vivo in immature male rats.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blake J., Li C. H. Human somatotropin. 55. Synthesis and growth-promoting activity of peptide analogs of the carboxyl terminal plasmin fragment. Int J Pept Protein Res. 1978 May;11(5):315–322. [PubMed] [Google Scholar]
  2. Blake J., Li C. H. The synthesis and biological activity of (165, 182, 189-S-carbamidomethylcysteine)-human growth hormone-(140-191). Int J Pept Protein Res. 1975;7(6):495–501. doi: 10.1111/j.1399-3011.1975.tb02471.x. [DOI] [PubMed] [Google Scholar]
  3. Hagenmaier H., Frank H. Increased coupling yields in solid phase peptide synthesis with a modified carbodiimide coupling procedure. Hoppe Seylers Z Physiol Chem. 1972 Dec;353(12):1973–1976. [PubMed] [Google Scholar]
  4. Kato K., Sairam M. R., Ramasharma K. Effect of porcine follicular fluid preparations on gonadotrophin secretion by the mouse pituitary gland in vitro. J Endocrinol. 1983 Jan;96(1):73–84. doi: 10.1677/joe.0.0960073. [DOI] [PubMed] [Google Scholar]
  5. Li C. H., Yamashiro D., Tseng L. F., Loh H. H. Synthesis and analgesic activity of human beta-endorphin. J Med Chem. 1977 Mar;20(3):325–328. doi: 10.1021/jm00213a001. [DOI] [PubMed] [Google Scholar]
  6. McCullagh D. R. DUAL ENDOCRINE ACTIVITY OF THE TESTES. Science. 1932 Jul 1;76(1957):19–20. doi: 10.1126/science.76.1957.19. [DOI] [PubMed] [Google Scholar]
  7. Ramasharma K., Sairam M. R. Isolation and characterization of inhibin from human seminal plasma. Ann N Y Acad Sci. 1982;383:307–328. doi: 10.1111/j.1749-6632.1982.tb23175.x. [DOI] [PubMed] [Google Scholar]
  8. Ramasharma K., Sairam M. R., Ranganathan M. R. Effect of inhibin like factors on gonadotrophin release by the mouse pituitary in vitro. Acta Endocrinol (Copenh) 1981 Dec;98(4):496–505. doi: 10.1530/acta.0.0980496. [DOI] [PubMed] [Google Scholar]
  9. Ramasharma K., Sairam M. R., Seidah N. G., Chrétien M., Manjunath P., Schiller P. W., Yamashiro D., Li C. H. Isolation, structure, and synthesis of a human seminal plasma peptide with inhibin-like activity. Science. 1984 Mar 16;223(4641):1199–1202. doi: 10.1126/science.6422553. [DOI] [PubMed] [Google Scholar]
  10. Seidah N. G., Ramasharma K., Sairam M. R., Chrétien M. Partial amino acid sequence of a human seminal plasma peptide with inhibin-like activity. FEBS Lett. 1984 Feb 13;167(1):98–102. doi: 10.1016/0014-5793(84)80840-6. [DOI] [PubMed] [Google Scholar]
  11. Yamashiro D. High-performance partition chromatography of peptides on a new cross-linked agarose. Int J Pept Protein Res. 1983 Sep;22(3):381–383. doi: 10.1111/j.1399-3011.1983.tb02106.x. [DOI] [PubMed] [Google Scholar]
  12. de Jong F. H. Inhibin--fact or artifact. Mol Cell Endocrinol. 1979 Jan;13(1):1–10. doi: 10.1016/0303-7207(79)90071-6. [DOI] [PubMed] [Google Scholar]

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