Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1984 Sep;81(17):5489–5493. doi: 10.1073/pnas.81.17.5489

Mouse kidney and submaxillary gland renin genes differ in their 5' putative regulatory sequences.

J J Panthier, M Dreyfus, T L Roux, F Rougeon
PMCID: PMC391731  PMID: 6089205

Abstract

The genomic DNAs that specify the renin mRNAs found in the submaxillary gland and in the kidney of Swiss mice have been isolated by molecular cloning in phage lambda. The Ren1 and Ren2 genes, encoding the kidney and submaxillary renins, respectively, span about 9.5 kilobases each. Restriction maps of both renin genes and their flanking sequences are presented. To correlate the differential expression of the renin genes with their structures, a sequence analysis of the putative 5' regulatory sequences of both genes was conducted. The results show that the leader sequences and promoter regions of both genes are highly homologous to a point located 179 nucleotides upstream from the transcription start point; the sequences diverge beyond this position.

Full text

PDF
5489

Images in this article

5492Image
on p.5492

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auffray C., Rougeon F. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA. Eur J Biochem. 1980 Jun;107(2):303–314. doi: 10.1111/j.1432-1033.1980.tb06030.x. [DOI] [PubMed] [Google Scholar]
  2. Benton W. D., Davis R. W. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. doi: 10.1126/science.322279. [DOI] [PubMed] [Google Scholar]
  3. Biggin M. D., Gibson T. J., Hong G. F. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963–3965. doi: 10.1073/pnas.80.13.3963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chandler V. L., Maler B. A., Yamamoto K. R. DNA sequences bound specifically by glucocorticoid receptor in vitro render a heterologous promoter hormone responsive in vivo. Cell. 1983 Jun;33(2):489–499. doi: 10.1016/0092-8674(83)90430-0. [DOI] [PubMed] [Google Scholar]
  5. Compton J. G., Schrader W. T., O'Malley B. W. DNA sequence preference of the progesterone receptor. Proc Natl Acad Sci U S A. 1983 Jan;80(1):16–20. doi: 10.1073/pnas.80.1.16. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dean D. C., Knoll B. J., Riser M. E., O'Malley B. W. A 5'-flanking sequence essential for progesterone regulation of an ovalbumin fusion gene. Nature. 1983 Oct 6;305(5934):551–554. doi: 10.1038/305551a0. [DOI] [PubMed] [Google Scholar]
  7. Gecse A., Wilson C. M., Erdös E. G. Induction of particle-bound renin and arginine esterase by testosterone in the mouse. Biochem Pharmacol. 1976 Apr 1;25(7):763–768. doi: 10.1016/0006-2952(76)90143-x. [DOI] [PubMed] [Google Scholar]
  8. Gillies S. D., Morrison S. L., Oi V. T., Tonegawa S. A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene. Cell. 1983 Jul;33(3):717–728. doi: 10.1016/0092-8674(83)90014-4. [DOI] [PubMed] [Google Scholar]
  9. Grez M., Land H., Giesecke K., Schütz G., Jung A., Sippel A. E. Multiple mRNAs are generated from the chicken lysozyme gene. Cell. 1981 Sep;25(3):743–752. doi: 10.1016/0092-8674(81)90182-3. [DOI] [PubMed] [Google Scholar]
  10. Groner B., Hynes N. E. Number and location of mouse mammary tumor virus proviral DNA in mouse DNA of normal tissue and of mammary tumors. J Virol. 1980 Mar;33(3):1013–1025. doi: 10.1128/jvi.33.3.1013-1025.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Heidmann O., Rougeon F. Multiple sequences related to a constant-region kappa light chain gene in the rabbit genome. Cell. 1982 Mar;28(3):507–513. doi: 10.1016/0092-8674(82)90205-7. [DOI] [PubMed] [Google Scholar]
  12. Hohn B., Murray K. Packaging recombinant DNA molecules into bacteriophage particles in vitro. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3259–3263. doi: 10.1073/pnas.74.8.3259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Holm I., Ollo R., Panthier J. J., Rougeon F. Evolution of aspartyl proteases by gene duplication: the mouse renin gene is organized in two homologous clusters of four exons. EMBO J. 1984 Mar;3(3):557–562. doi: 10.1002/j.1460-2075.1984.tb01846.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Huang A. L., Ostrowski M. C., Berard D., Hager G. L. Glucocorticoid regulation of the Ha-MuSV p21 gene conferred by sequences from mouse mammary tumor virus. Cell. 1981 Dec;27(2 Pt 1):245–255. doi: 10.1016/0092-8674(81)90408-6. [DOI] [PubMed] [Google Scholar]
  15. Hurst H. C., Parker M. G. Rat prostatic steroid binding protein: DNA sequence and transcript maps of the two C3 genes. EMBO J. 1983;2(5):769–774. doi: 10.1002/j.1460-2075.1983.tb01498.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hynes N., van Ooyen A. J., Kennedy N., Herrlich P., Ponta H., Groner B. Subfragments of the large terminal repeat cause glucocorticoid-responsive expression of mouse mammary tumor virus and of an adjacent gene. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3637–3641. doi: 10.1073/pnas.80.12.3637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kelly K., Cochran B. H., Stiles C. D., Leder P. Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor. Cell. 1983 Dec;35(3 Pt 2):603–610. doi: 10.1016/0092-8674(83)90092-2. [DOI] [PubMed] [Google Scholar]
  18. Lee F., Mulligan R., Berg P., Ringold G. Glucocorticoids regulate expression of dihydrofolate reductase cDNA in mouse mammary tumour virus chimaeric plasmids. Nature. 1981 Nov 19;294(5838):228–232. doi: 10.1038/294228a0. [DOI] [PubMed] [Google Scholar]
  19. Maniatis T., Hardison R. C., Lacy E., Lauer J., O'Connell C., Quon D., Sim G. K., Efstratiadis A. The isolation of structural genes from libraries of eucaryotic DNA. Cell. 1978 Oct;15(2):687–701. doi: 10.1016/0092-8674(78)90036-3. [DOI] [PubMed] [Google Scholar]
  20. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  21. McMaster G. K., Beard P., Engers H. D., Hirt B. Characterization of an immunosuppressive parvovirus related to the minute virus of mice. J Virol. 1981 Apr;38(1):317–326. doi: 10.1128/jvi.38.1.317-326.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Messing J., Crea R., Seeburg P. H. A system for shotgun DNA sequencing. Nucleic Acids Res. 1981 Jan 24;9(2):309–321. doi: 10.1093/nar/9.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Mullins J. J., Burt D. W., Windass J. D., McTurk P., George H., Brammar W. J. Molecular cloning of two distinct renin genes from the DBA/2 mouse. EMBO J. 1982;1(11):1461–1466. doi: 10.1002/j.1460-2075.1982.tb01338.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Mulvihill E. R., LePennec J. P., Chambon P. Chicken oviduct progesterone receptor: location of specific regions of high-affinity binding in cloned DNA fragments of hormone-responsive genes. Cell. 1982 Mar;28(3):621–632. doi: 10.1016/0092-8674(82)90217-3. [DOI] [PubMed] [Google Scholar]
  25. Panthier J. J., Foote S., Chambraud B., Strosberg A. D., Corvol P., Rougeon F. Complete amino acid sequence and maturation of the mouse submaxillary gland renin precursor. Nature. 1982 Jul 1;298(5869):90–92. doi: 10.1038/298090a0. [DOI] [PubMed] [Google Scholar]
  26. Panthier J. J., Holm I., Rougeon F. The mouse Rn locus: S allele of the renin regulator gene results from a single structural gene duplication. EMBO J. 1982;1(11):1417–1421. doi: 10.1002/j.1460-2075.1982.tb01332.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Payvar F., DeFranco D., Firestone G. L., Edgar B., Wrange O., Okret S., Gustafsson J. A., Yamamoto K. R. Sequence-specific binding of glucocorticoid receptor to MTV DNA at sites within and upstream of the transcribed region. Cell. 1983 Dec;35(2 Pt 1):381–392. doi: 10.1016/0092-8674(83)90171-x. [DOI] [PubMed] [Google Scholar]
  28. Piccini N., Knopf J. L., Gross K. W. A DNA polymorphism, consistent with gene duplication, correlates with high renin levels in the mouse submaxillary gland. Cell. 1982 Aug;30(1):205–213. doi: 10.1016/0092-8674(82)90026-5. [DOI] [PubMed] [Google Scholar]
  29. Renkawitz R., Beug H., Graf T., Matthias P., Grez M., Schütz G. Expression of a chicken lysozyme recombinant gene is regulated by progesterone and dexamethasone after microinjection into oviduct cells. Cell. 1982 Nov;31(1):167–176. doi: 10.1016/0092-8674(82)90416-0. [DOI] [PubMed] [Google Scholar]
  30. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  31. Rougeon F., Chambraud B., Foote S., Panthier J. J., Nageotte R., Corvol P. Molecular cloning of a mouse submaxillary gland renin cDNA fragment. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6367–6371. doi: 10.1073/pnas.78.10.6367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Rougeon F., Mach B. Cloning and amplification of rabbit alpha- and beta-globin gene sequences into Escherichia coli plasmids. J Biol Chem. 1977 Apr 10;252(7):2209–2217. [PubMed] [Google Scholar]
  33. Scheidereit C., Geisse S., Westphal H. M., Beato M. The glucocorticoid receptor binds to defined nucleotide sequences near the promoter of mouse mammary tumour virus. Nature. 1983 Aug 25;304(5928):749–752. doi: 10.1038/304749a0. [DOI] [PubMed] [Google Scholar]
  34. Smith T. F., Waterman M. S., Fitch W. M. Comparative biosequence metrics. J Mol Evol. 1981;18(1):38–46. doi: 10.1007/BF01733210. [DOI] [PubMed] [Google Scholar]
  35. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  36. Ucker D. S., Ross S. R., Yamamoto K. R. Mammary tumor virus DNA contains sequences required for its hormone-regulated transcription. Cell. 1981 Dec;27(2 Pt 1):257–266. doi: 10.1016/0092-8674(81)90409-8. [DOI] [PubMed] [Google Scholar]
  37. Walker M. D., Edlund T., Boulet A. M., Rutter W. J. Cell-specific expression controlled by the 5'-flanking region of insulin and chymotrypsin genes. Nature. 1983 Dec 8;306(5943):557–561. doi: 10.1038/306557a0. [DOI] [PubMed] [Google Scholar]
  38. Walker P., Brown-Luedi M., Germond J. E., Wahli W., Meijlink F. C., van het Schip A. D., Roelink H., Gruber M., Ab G. Sequence homologies within the 5' end region of the estrogen-controlled vitellogenin gene in Xenopus and chicken. EMBO J. 1983;2(12):2271–2279. doi: 10.1002/j.1460-2075.1983.tb01734.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Wilson C. M., Cherry M., Taylor B. A., Wilson J. D. Genetic and endocrine control of renin activity in the submaxillary gland of the mouse. Biochem Genet. 1981 Jun;19(5-6):509–523. doi: 10.1007/BF00484623. [DOI] [PubMed] [Google Scholar]
  40. Wilson C. M., Erdös E. G., Wilson J. D., Taylor B. A. Location on chromosome 1 of Rnr, a gene that regulates renin in the submaxillary gland of the mouse. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5623–5626. doi: 10.1073/pnas.75.11.5623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Wilson C. M., Taylor B. A. Genetic regulation of thermostability of mouse submaxillary gland renin. J Biol Chem. 1982 Jan 10;257(1):217–223. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES