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. 1984 Sep;81(17):5519–5522. doi: 10.1073/pnas.81.17.5519

In vitro transcription of a silkworm 5S RNA gene requires an upstream signal.

D G Morton, K U Sprague
PMCID: PMC391737  PMID: 6089209

Abstract

The RNA polymerase III transcription complex from Bombyx mori silkworms, in contrast to that from Xenopus frogs, requires a signal that is upstream from the transcription initiation site. We have found that part of the normal 5' flanking sequence is essential for transcription of Bombyx 5S RNA genes by homologous cell-free extracts. The critical region is structurally similar to the upstream signal that directs Bombyx tRNA gene transcription. The association of such a control element with two classes of polymerase III templates argues that it acts through a general component of the Bombyx transcription apparatus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bogenhagen D. F., Sakonju S., Brown D. D. A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region. Cell. 1980 Jan;19(1):27–35. doi: 10.1016/0092-8674(80)90385-2. [DOI] [PubMed] [Google Scholar]
  2. Ciliberto G., Raugei G., Costanzo F., Dente L., Cortese R. Common and interchangeable elements in the promoters of genes transcribed by RNA polymerase iii. Cell. 1983 Mar;32(3):725–733. doi: 10.1016/0092-8674(83)90058-2. [DOI] [PubMed] [Google Scholar]
  3. Dingermann T., Burke D. J., Sharp S., Schaack J., Söll D. The 5- flanking sequences of Drosophila tRNAArg genes control their in vitro transcription in a Drosophila cell extract. J Biol Chem. 1982 Dec 25;257(24):14738–14744. [PubMed] [Google Scholar]
  4. Fournier A., Guérin M. A., Corlet J., Clarkson S. G. Structure and in vitro transcription of a glycine tRNA gene from Bombyx mori. EMBO J. 1984 Jul;3(7):1547–1552. doi: 10.1002/j.1460-2075.1984.tb02009.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Galibert F., Sedat J., Ziff E. Direct determination of DNA nucleotide sequences: structure of a fragment of bacteriophage phiX172 DNA. J Mol Biol. 1974 Aug 15;87(3):377–407. doi: 10.1016/0022-2836(74)90093-x. [DOI] [PubMed] [Google Scholar]
  6. Larson D., Bradford-Wilcox J., Young L. S., Sprague K. U. A short 5' flanking region containing conserved sequences is required for silkworm alanine tRNA gene activity. Proc Natl Acad Sci U S A. 1983 Jun;80(11):3416–3420. doi: 10.1073/pnas.80.11.3416. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Legerski R. J., Hodnett J. L., Gray H. B., Jr Extracellular nucleases of pseudomonas BAL 31. III. Use of the double-strand deoxyriboexonuclease activity as the basis of a convenient method for the mapping of fragments of DNA produced by cleavage with restriction enzymes. Nucleic Acids Res. 1978 May;5(5):1445–1464. doi: 10.1093/nar/5.5.1445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  9. Messing J., Vieira J. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982 Oct;19(3):269–276. doi: 10.1016/0378-1119(82)90016-6. [DOI] [PubMed] [Google Scholar]
  10. Morton D. G., Sprague K. U. Silkworm 5S RNA and alanine tRNA genes share highly conserved 5' flanking and coding sequences. Mol Cell Biol. 1982 Dec;2(12):1524–1531. doi: 10.1128/mcb.2.12.1524. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Peterson R. C., Doering J. L., Brown D. D. Characterization of two xenopus somatic 5S DNAs and one minor oocyte-specific 5S DNA. Cell. 1980 May;20(1):131–141. doi: 10.1016/0092-8674(80)90241-x. [DOI] [PubMed] [Google Scholar]
  12. Raymond G. J., Johnson J. D. The role of non-coding DNA sequences in transcription and processing of a yeast tRNA. Nucleic Acids Res. 1983 Sep 10;11(17):5969–5988. doi: 10.1093/nar/11.17.5969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sakonju S., Bogenhagen D. F., Brown D. D. A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region. Cell. 1980 Jan;19(1):13–25. doi: 10.1016/0092-8674(80)90384-0. [DOI] [PubMed] [Google Scholar]
  14. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sharp S., Dingermann T., Söll D. The minimum intragenic sequences required for promotion of eukaryotic tRNA gene transcription. Nucleic Acids Res. 1982 Sep 25;10(18):5393–5406. doi: 10.1093/nar/10.18.5393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sprague K. U., Larson D., Morton D. 5' flanking sequence signals are required for activity of silkworm alanine tRNA genes in homologous in vitro transcription systems. Cell. 1980 Nov;22(1 Pt 1):171–178. doi: 10.1016/0092-8674(80)90165-8. [DOI] [PubMed] [Google Scholar]
  17. Wu G. J., Zubay G. Prolonged transcription in a cell-free system involving nuclei and cytoplasm. Proc Natl Acad Sci U S A. 1974 May;71(5):1803–1807. doi: 10.1073/pnas.71.5.1803. [DOI] [PMC free article] [PubMed] [Google Scholar]

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