Abstract
Ring glands or brain—ring gland complexes from third-instar Drosophila melanogaster larvae secreted ecdysone [(22R)-2β,3β,14α,22,25-pentahydroxy-5β-cholest-7-en-6-one] and two less polar ecdysteroids (LP1 and LP2) in vitro. Radioimmunoassay with different antisera indicated that LP1 and LP2 are side-chain-modified analogues of ecdysone. In high-performance liquid chromatography, the retention time of LP2 was equivalent to that of a precursor of makisterone A [(20R,22R)-2β,3β,14α,20,22,25-hexahydroxy-24-methyl-5β-cholest-7-en-6-one] secreted by Dysdercus fasciatus prothoracic glands in vitro. LP2 was metabolized in vitro by the fat body of Drosophila larvae to a product with the characteristics of makisterone A when analyzed by gas chromatography/mass spectrometry (selected ion monitoring). Evidence was obtained for the presence of makisterone A in Drosophila larvae. These data suggest that LP2 is 20-deoxymakisterone A (24-methylecdysone) and that makisterone A could function as an additional moulting hormone in Drosophila, although 20-deoxymakisterone A production is apparently dependent on the sterol composition of the diet. LP1 has not been identified.
Keywords: ecdysteroid secretion, phytosterols
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- Bollenbacher W. E., Goodman W., Vedeckis W. V., Gilbert L. I. The in vitro synthesis and secretion of alpha-ecdysone by the ring glands of the fly, Sarcophaga bullata. Steroids. 1976 Mar;27(3):309–324. doi: 10.1016/0039-128x(76)90053-2. [DOI] [PubMed] [Google Scholar]
- Borst D. W., O'Connor J. D. Trace analysis of ecdysones by gas-liquid chromatography, radioimmunoassay and bioassay. Steroids. 1974 Nov;24(5):637–656. doi: 10.1016/0039-128x(74)90017-8. [DOI] [PubMed] [Google Scholar]
- Bos M., Burnet B., Farrow R., Woods R. A. Development of Drosophila on sterol mutants of the yeast Saccharomyces cerevisiae. Genet Res. 1976 Oct;28(2):163–176. doi: 10.1017/s0016672300016840. [DOI] [PubMed] [Google Scholar]
- Cooke J., Sang J. H. Utilization of sterols by larvae of Drosophila melanogaster. J Insect Physiol. 1970 May;16(5):801–812. doi: 10.1016/0022-1910(70)90214-3. [DOI] [PubMed] [Google Scholar]
- Horn D. H., Wilkie J. S., Sage B. A., O'Conner J. D. A high affinity antiserum specific for the ecdysone nucleus. J Insect Physiol. 1976;22(6):901–905. doi: 10.1016/0022-1910(76)90263-8. [DOI] [PubMed] [Google Scholar]
- King D. S., Bollenbacher W. E., Borst D. W., Vedeckis W. V., O'connor J. D., Ittycheriah P. I., Gilbert L. I. The Secretion of alpha-Ecdysone by the Prothoracic Glands of Manduca sexta In Vitro. Proc Natl Acad Sci U S A. 1974 Mar;71(3):793–796. doi: 10.1073/pnas.71.3.793. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mendis A. H., Rose M. E., Rees H. H., Goodwin T. W. Ecdysteroids in adults of the nematode, Dirofilaria immitis. Mol Biochem Parasitol. 1983 Nov;9(3):209–226. doi: 10.1016/0166-6851(83)90098-1. [DOI] [PubMed] [Google Scholar]
- Porcheron P., Foucrier J., Gros C., Pradelles P., Cassier P., Dray F. Radioimmunoassay of arthropod moulting hormone:beta-ecdysone antibodies production and 125 I-iodinated tracer preparation. FEBS Lett. 1976 Jan 15;61(2):159–162. doi: 10.1016/0014-5793(76)81027-7. [DOI] [PubMed] [Google Scholar]
- Soumoff C., Horn D. H., O'Connor J. D. Production of a new antiserum to arthropod molting hormone and comparison with two other antisera. J Steroid Biochem. 1981 May;14(5):429–435. doi: 10.1016/0022-4731(81)90353-8. [DOI] [PubMed] [Google Scholar]
- Spindler K. D., Beckers C., Gröschel-Stewart U., Emmerich H. A radioimmunoassay for arthropod moulting hormones, introducing a novel method of immunogen coupling. Hoppe Seylers Z Physiol Chem. 1978 Oct;359(10):1269–1272. doi: 10.1515/bchm2.1978.359.2.1269. [DOI] [PubMed] [Google Scholar]